Abstract
AUTORADIOGRAPHIC work in various laboratories on synthesis of nucleic acids in the intact cell has indicated that nuclear RNA is a precursor for cytoplasmic RNA, although different authors have placed different emphasis on the role of the nucleolus and chromatin portion1–5. It was suggested that RNA mediates incorporation of pyrimidine nucleotides into DNA, probably through an intermediate fraction7,8. In testing and extending these observations, the uptake of tritiated cytidine, uridine, thymidine and deoxyuridine into the various nueleotide fractions was examined for dependence on RNA-ase and DNA-ase, added to the cells in culture before, and at the same time as, exposure to the tritiated precursors.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Goldstein, L., and Micou, J., J. Biophys. Biochem. Cytol., 6, 1 (1959).
Woods, P. S., and Taylor, J. H., Lab. Invest., 8, 309 (1959).
Fitzgerald, P. J., and Vinijchaikul, K., Lab. Invest., 8, 319 (1959).
Amano, M., and Leblond, C. P., Exp. Cell Res., 20, 250 (1960).
Feinendegen, L. E., in Structure and Function of Genetic Elements, Brookhaven Symp. in Biology, No. 12, 172 (1959).
Feinendegen, L. E., Bond, V. P., Shreeve, W. W., and Painter, R. B., Exp. Cell Res., 19, 443 (1960).
Feinendegen, L. E., Bond, V. P., and Painter, R. B., Exp. Cell Res., 22, 381 (1961).
Feinendegen, L. E., Bond, V. P., and Hughes, W. L., presented at the first Intern. Congress of Histochemistry and Cytochemistry, Paris (1960).
Ledoux, L., and Revell, S. H., Biochim. Biophys. Acta., 18, 416 (1955).
Brachet, J., Biochem. Cytol., 264 (Academic Press, 1957).
Novikoff, A. B., Ryan, J., and Pobder, E., J. Histochem. Cytochem., 2, 401 (1954).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
FEINENDEGEN, L., BOND, V. & DREW, R. Effect of Ribonuclease and Deoxyribonuclease on Incorporation of Tritiated Pyrimidine-Nucleosides into Ribonucleic Acid and Deoxyribonucleic Acid in Human Cancer Cells (HeLa) in Culture. Nature 191, 1398–1399 (1961). https://doi.org/10.1038/1911398a0
Issue Date:
DOI: https://doi.org/10.1038/1911398a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
