Migration-elution of Zones in an Electrophoresis Column

Abstract

SO far the preparation of electrophoretically defined fractions by means of zone electrophoresis in powder columns has been performed in two separate steps. Electrophoresis has been succeeded by displacement of the solution in the column^{1 2} or the entire contents of the column have been removed from the tube and cut in segments from which the substance has been recovered by washing^3,4. Both methods obviously suffer from a number of drawbacks. The latter technique is rather time-consuming and is especially complicated when applied on a large scale (100-ml. sample or more). The former method is simple in operation but its main disadvantage is its dependence on the filtering properties of the powder. As Kunkel⁴ has pointed out, the displacement of zones after electrophoresis will to some extent impair the separation already achieved at the end of the first step.