Abstract
IN a communication under this title in Nature of April 13, Mr. K. Gundersen concludes that preservation under paraffin oil seems to be more promising than any other method used. He was evidently not aware that I mentioned in an article in Nature of February 12, 1955, the use of mineral oil for the maintenance of crude and pure cultures of nitrifying bacteria and briefly discussed the results of my experiments in a paper in the annual report of the Experimental and Research Station, Cheshunt, for 1954. Mr. Gundersen did not obtain the results he expected from this method because he did not give his cultures a chance to develop, but put them under paraffin oil immediately after inoculation. My cultures, which were only partly purified, were fully developed and showed peak reactions for nitrite and nitrate, respectively, before they were covered with paraffin oil. They were then kept at room temperature in a cupboard for from three to seven months. Subcultures made after these times developed into fully active cultures; however, the speed of their development differed, probably depending on the strains and the impurities present.
Similar content being viewed by others
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
KLEIN, R. Preservation of Nitrosomonas . Nature 179, 1200 (1957). https://doi.org/10.1038/1791200a0
Issue Date:
DOI: https://doi.org/10.1038/1791200a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.