Abstract
WHENEVER it has been possible to test unambiguously the relationship between chiasmata and crossing over, they have been found to coincide in time and place. Evidence concerning the time of crossing over indicates that chiasma formation and genetic recombination do not occur during premeiotic DNA synthesis. Various physical and chemical agents affect recombination and chiasma formation and treatment is effective during the S phase (DNA synthesis) before leptotene and during the meiotic DNA synthesis at zygotene–pachytene. Treatment with mitomycin C and γ-rays during the S phase before leptotene has always caused a decline in chiasma frequency or recombination except in Chlamydomonas1. An increase of chiasma frequency or recombination has been found as a rule after treatment with deoxyadenosine at zygotene, again except with Chlamydomonas, when there was a decline in recombination. All these agents have been presumed primarily to affect DNA synthesis. The presence of cycloheximide at zygotene–pachytene prevents the formation of chiasmata2, which also happens when Trillium meiocytes are explantcd into culture at leptotene.
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SEN, S. Regulation of Chiasma Frequency in Lilium Microsporocytes in vitro. Nature 224, 178–179 (1969). https://doi.org/10.1038/224178a0
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DOI: https://doi.org/10.1038/224178a0
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