Abstract
THE preparation of subcellular myelin-rich fractions so far has been largely restricted to the central nervous system (brain, cord and optic nerve), whereas separation of myelin from peripheral nerve has been beset with the problem of homogenizing the neural components in a morass of endoneurial and epineurial collagen. On attempting to homogenize peripheral nerves, the collagen fibres roll up into a slithery sludge, from which the myelin cannot be easily released and separated on a density gradient. Thus Horrocks1 admitted that the yield of myelin from peripheral nerves is much lower than that from the cord and he reported that “a substantial residue remains undispersed”.
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ADAMS, C., ABDULLA, Y., TURNER, D. et al. Subcellular Preparation of Peripheral Nerve Myelin. Nature 220, 171–173 (1968). https://doi.org/10.1038/220171a0
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DOI: https://doi.org/10.1038/220171a0
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