Abstract
OVULATION in Xenopus laevis can be induced by injecting human chorionic gonadotrophin (HCG); this procedure has been used to diagnose pregnancy1,2, or for the biological assay of HCG. Either the proportion of animals ovulating in a group, or the number of eggs laid by each individual, may form the basis of a test3–5. A dose of 90 international units (IU) of HCG causes ovulation in most Xenopus, although there is a considerable variation in the number of eggs laid by each individual, from a few tens to several thousands. A method to estimate the number of eggs laid is described here.
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BARR, W., HOBSON, B. & DI VITA, G. Method for estimating the Number of Eggs laid by Xenopus laevis in Response to the Injection of Gonadotrophin. Nature 214, 827–828 (1967). https://doi.org/10.1038/214827a0
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DOI: https://doi.org/10.1038/214827a0
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