Abstract
WE have investigated the influence of 4-benzyloxy-3,5-diiodo-benzoic acid (BIBA) on the oxidative phosphoryl-ation of isolated rat liver mitochondria. The work continues previous investigations from our laboratory regarding the effects of chlorpromazine1, librium2, L-3,5,3′-triiodo-thyronine3,4 on the oxidative phosphorylation of liver and tumour mitochondria. BIBA is known as a competitive antagonist to thyroxine and structurally related compounds5. Brody6 suggests that this substance uncouples the oxidative phosphorylation in a similar way to 2,4-dinitrophenol (DNP) or barbiturates. Studying the mode of action on mitochondria BIBA was compared with amytal, which is a specific inhibitor at the flavo-protein site of the mitochondrial DPNH-cytochrome c-reductase system7. Evidence is given here that BIBA uncouples phosphorylation from respiration in isolated liver mitochondria, and we have tried to find the site of action of this uncoupling agent.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Lührs, W., Bacigalupo, G., Kadenbach, B., and Heise, E., Experientia, 15, 376 (1959).
Kadenbach, B., and Lührs, W., Nature, 192, 174 (1961).
Lührs, W., Heise, E., and Bacigalupo, G., Nature, 183, 1534 (1959).
Bacigalupo, G., Lührs, W., and Heise, E., Acta Biol. Med. Germ., 2/5, 455 (1959).
Frieden, E., and Winzler, R. J., J. Biol. Chem., 179, 423 (1949).
Brody, Th. M., Pharm. Rev., 7/3, 335 (1955).
Chance, B., Enzyme Units of Biological Structure and Function, 447 (Academic Press, Inc., New York, 1956).
Ernster, L., and Löw, H., Exp. Cell Res., Supp. 3, 133 (1955).
Emmelot, P., Bos, C. J., and Reyers, J. H. M., Z. Krebsforsch., 64, 22 (1960).
Fiske, C. H., and Subbarow, Y., Methods in Enzymology, 3, 843 (1957).
Lehninger, A. L., J. Biol. Chem., 234, 2465 (1959).
Raaflaub, I., Helv. Chim. Acta, 38, 27 (1955).
Lardy, H. A., and Wellman, H., J. Biol. Chem., 195, 215 (1952).
Siekevitz, P., Löw, H., Ernster, L., and Lindberg, O., Biochim. Biophys. Acta, 29, 378 (1958).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
WAND, H., BACIGALUPO, G. Effects of 4-Benzyloxy-3,5-diiodo-benzoic Acid on the Oxidative Phosphorylation of Isolated Mitochondria. Nature 202, 295–296 (1964). https://doi.org/10.1038/202295a0
Issue Date:
DOI: https://doi.org/10.1038/202295a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.