Abstract
THE dependence on concentration of the hydrodynamic properties of DNA solutions has been the subject of some recent publications1–4. It has been shown in particular1, that under specified conditions the dependence on concentration of the reduced viscosity and the sedimentation coefficient of aqueous DNA solutions may be expressed by empirical formulæ that are formally analogous to those found to apply for most uncharged polymer solutions, namely: In these equations the symbols s and s0 represent the sedimentation coefficients at concentration c and zero respectively. ηsp/c and [η] are the corresponding reduced viscosity values and α and k are dimensionless constants.
Similar content being viewed by others
Article PDF
References
Eigner, J., Schildkraut, C., and Doty, P., Biochim. Biophys. Acta, 55, 13 (1962).
Ganguli, P. K., and Bhattacharyya, A. L., Nature, 196, 1201 (1962).
Ganguli, P. K., Nature, 200, 164 (1963).
Kurucsev, T., Arch. Biochem. Biophys., 102, 120 (1968).
Schachman, H. K., Ultracentrifugation in Biochemistry, 90 (Academic Press, Inc., New York, 1959).
Ref. 5, 225.
Cragg, L. H., and Bigelow, C. C., J. Polymer Sci., 16, 177 (1955).
Harmsen, G. J., Van Schooten, J., and Overbeek, J. T. G., J. Colloid Sci., 10, 315 (1955).
Goring, D. A. I., and Rezanovich, A., J. Colloid Sci., 15, 472 (1960).
Darskus, R. L., Jordan, D. O., Kurucsev, T., and Martin, M. L. (to be published).
Goring, D. A. I., and Sitaramaiah, G., Polymer, 4, 7 (1963).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
DARSKUS, R., JORDAN, D. & KURUCSEV, T. Concentration Effects in Measurements of Sedimentation Velocity and Viscosity of Deoxyribonucleic Acid Solutions. Nature 201, 1215 (1964). https://doi.org/10.1038/2011215a0
Issue Date:
DOI: https://doi.org/10.1038/2011215a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.