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  • 2025-2025
  • 2000-2004
  • 1975-1979  (8)
  • 1965-1969
  • 1935-1939
  • 1910-1914
  • 1850-1859
  • 1840-1849
  • 1976  (8)
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  • American fiction., lcsh
  • Immunohistochemistry
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Datenquelle
Materialart
Erscheinungszeitraum
  • 2025-2025
  • 2000-2004
  • 1975-1979  (8)
  • 1965-1969
  • 1935-1939
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Jahr
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  • 1
    Digitale Medien
    Digitale Medien
    Fluorescamine fluorescence detection of growth hormone-producing cells in human pituitary adenomas (1976)
    Springer
    Virchows Archiv 371 (1976), S. 177-181 
    Details
    ISSN: 1432-2307
    Schlagwort(e): Fluorescamine fluorescence ; Immunohistochemistry ; Growth hormone ; Pituitary adenomas
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Formalin-fixed and paraplast-embedded tissue specimens of human pituitary, thyroid, and pancreas were investigated using fluorescamine fluorescence and immunohistochemical methods. Growth hormone-producing cells present in normal and neoplastic pituitary tissue exhibited fluorescamine fluorescence. The other tissues examined showed no fluorescamine binding.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00433066
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  • 2
    Digitale Medien
    Digitale Medien
    Uteroglobin in the rabbit (1976)
    Springer
    Cell & tissue research 170 (1976), S. 415-424 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Uterine specific proteins ; Oviduct ; Uterus ; Blastocyst of the Rabbit ; Immunohistochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The localization and release of uteroglobin (UGL) were investigated immunohistologically in the oviducts and uteri of female rabbits from oestrus through the 7th day post coitum and the blastocyst on the 7th day post coitum. UGL was detected within Fallopian tube cells even during oestrus. Granules of UGL appeared toward the bases of these cells. Subsequently, the cells became almost entirely filled with UGL. Drop-like protrusions of the apical cytoplasm suggest a mechanism of apocrine extrusion. All stages of filling and extrusion were visible during the entire preimplantation period. During oestrus, synthesis of UGL within uterine cells becomes sufficiently advanced so that extrusion has either already begun or is about to begin. UGL positive material first appears in the supranuclear regions. Later the entire cytoplasm shows a positive reaction. An uneven distribution of UGL cells is observed in the endometrium. Since only the glands adjacent to the myometrium and cells of the cavum epithelium contain UGL, a striking mosaic of UGL positive and negative cells results. The present report is the first detecting UGL in single cells of the blastocyst. Both entodermal and ectodermal cells proved to be UGL positive. The synthesis and section of UGL in the oviduct and uterus and the possible origins of UGL in the blastocyst are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00219420
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  • 3
    Digitale Medien
    Digitale Medien
    Uteroglobin in the rabbit (1976)
    Springer
    Cell & tissue research 170 (1976), S. 425-434 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Uterine specific proteins ; Uterus of the rabbit ; Immunohistochemistry ; Oestrogen-Progesterone
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Rabbit uterine uteroglobin (UGL) was studied by electrophoretic and immunological methods following normal copulation, after ovariectomy and progesterone treatment, 17β-oestradiol and combined progesterone treatment, 17β-oestradiol treatment alone and after HCG-induced pseudopregnancy. Electrophoretic studies show the amount of ULG in uterine secretions, the immunological investigations indicate the intracellular localization of ULG and the distribution of ULG-positive cells in the endometrium. No obvious differences were found between the uteri 7 days after injection with chorion-gonadotropin and those 7 days following normal copulation. No differences could be demonstrated between the uteri of animals 35 days following ovariectomy and subsequent progesterone treatment on Days 31–33 and those of normal 7 d. post coitum (p.c.) animals. Uteri from animals treated with progesterone on Days 2–5 p.c. contained more ULG-positive cells than controls. 17β-oestradiol treatment with and without subsequent progesterone treatment resulted, in both gravid and ovariectomized animals, in the formation of a tall columnar endometrial epithelium. Treatment with 17β-oestradiol on Days 1 and 2 p.c. led to a decrease in the number of UGL-positive cells at 7 days p.c. Even after ovariectomy with 17β-oestradiol substitution, UGL-positive cells were still present in the endometrium. However a secretion of any magnitude could not be detected. The importance of differentiation between synthesis and secretion (= release) as distinct phases of the glandular response is especially emphasised by the latter findings.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00219421
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  • 4
    Digitale Medien
    Digitale Medien
    Immunohistochemical localization of LH-producing cells in the adenohypophysis of the Japanese quail, Coturnix coturnix japonica (1976)
    Springer
    Cell & tissue research 167 (1976), S. 453-460 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Adenohypophysis ; Japanese quail ; Immunohistochemistry ; LH producing cells
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The cells that produce luteinizing hormone (LH) in the adenohypophysis of the Japanese quail were identified immunohistochemically using anti-chicken LH serum and horseradish peroxidase-labeled goat anti-rabbit gamma globulin serum. The LH cells are localized in the caudal lobe of the pars distalis. They are elongate in shape and are polarized toward the sinusoids, especially in their active states. Alterations in size of LH cells are directly related to changes in circulating LH levels as induced by castration or photostimulation. The LH cells identified immunohistochemically were only stained by alcian blue with periodic acid-Schiff (PAS), alcian blue and orange G. PAS-positive gonadotropic cells in the cephalic lobe were stained immunohistochemically only slightly if at all using anti-chicken LH serum and consequently may be FSH producing cells. In the cephalic lobe another type of basophilic cell was stained with alcian blue. These cells were also stained immunohistochemically with anti-chicken LH serum. These cells may possibly be identified as TSH cells due to the characteristics of the antichicken LH serum used in this study which cross react with LH and TSH but only slightly with FSH, and also on the basis of previous light and electron microscopic studies.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00215177
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  • 5
    Digitale Medien
    Digitale Medien
    Immunohistochemical study on adenohypophysial primordia in organ culture (1976)
    Springer
    Cell & tissue research 166 (1976), S. 407-412 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Pituitary primordium ; Cytodifferentiation ; Rat ; Organ culture ; Immunohistochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Rathke's pouches isolated from rat fetuses on day 12 were maintained in organ culture for 9 days and investigated immunohistochemically to test whether or not the hypothalamus is involved in the cytodifferentiation of the adenohypophysis. The unlabeled antibody enzyme method demonstrated that the cultured tissue contains different types of glandular cells, i.e., adrenocorticotropin (ACTH)-, growth hormone (GH)-, luteinizing hormone (LH)-, thyrotropin (TSH)-, and prolactin-producing cells. Indirect evidence was also obtained to indicate the presence of melanocyte stimulating hormone (MSH)-cells. These findings suggest that adenohypophysial primordial cells of rats start to synthesize their respective hormones without stimuli from neurosecretory substances of the brain which are known to be essential for the maintenance of the secretory activity of the adult gland.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00220135
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  • 6
    Digitale Medien
    Digitale Medien
    Ultrastructural immunohistochemical localization of vasopressin in the hypothalamic-neurohypophysial system of three murids (1976)
    Springer
    Cell & tissue research 174 (1976), S. 69-81 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Vasopressin ; Immunohistochemistry ; Hypothalamus ; Hypophysis ; Murids
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Vasopressin was immunohistochemically localized at the electron microscopic (EM) level in the hypothalamic-neurohypophysial system (HNS) of three murids. Antiserum to vasopressin was produced in rabbits injected with lysine vasopressin (LVP) conjugated to egg albumin (EA), anti-EA being precipitated prior to staining. Sternberger's unlabeled antibody peroxidase technique was employed, immunoreactivity being designated by peroxidase anti-peroxidase (PAP) molecules and electron opacity. Immunoreactive neurosecretory granules (NSG) were found in the perikarya of the supraoptic nucleus (SON) in all three murids investigated, although far more profusely in the two wild strains. Immunoreactive axonal NSG were observed in the inner and outer zones of the median eminence (ME), and within most of the axons and terminals in the neurohypophysis. The concentration of primary serum effective for staining the SON (1∶10–1∶50) was far higher than that required for the ME and the neurohypophysis (1:500–1:1,200). AntiLVP also induced electron opacity of granules in cells of the pars intermedia (PI). Discussion centers on the significance of immunoreactive NSG in the neurosecretory (NS) perikarya, on the possibility of an extragranular pool of hormone, and on speculation about the electron opacity of the PI granules.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00222151
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  • 7
    Digitale Medien
    Digitale Medien
    Insulin-, glucagon-, and somatostatin-immunoreactive endocrine cells in the equine pancreas (1976)
    Springer
    Cell & tissue research 172 (1976), S. 447-454 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Pancreas ; Horse ; Immunohistochemistry ; Insulin ; Glucagon ; Somatostatin
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Equine pancreas was investigated with immunohistochemical methods to study the distribution of endocrine cells immunoreactive to antiinsulin, anti-glucagon, and anti-somatostatin. A-cells demonstrable by antiglucagon are located in the center of Langerhans islets and frequently in the duct epithelium. Few A-cells are seen associated to acini. Anti-insulin reactive B-cells form a large zone around the center of the Langerhans islets in which some B-cells lie between exocrine cells and others, although few, are located in the duct epithelium. D-cells stained with anti-somatostatin serum form a discontinuous outermost zone around the Langerhans islets. In some islets the D-cells are also observed among the B-cells or between the border of A- and B-cells. Single D-cells are seen in the duct epithelium or between acinar cells. In younger horses, endocrine cells are more frequently associated in bulges of the duct system. The histotopographic relation between these endocrine cell types is discussed with respect to its functional significance.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00220331
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  • 8
    Digitale Medien
    Digitale Medien
    35. Die konservative Behandlung kindlicher Frakturen (1976)
    Springer
    Langenbeck's archives of surgery 342 (1976), S. 287-290 
    Details
    ISSN: 1435-2451
    Schlagwort(e): Key words ; Fractures, juvenile ; Reduction, closed ; Konservative Behandlung kindlicher Frakturen
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Die vorwiegend konservative Behandlung kindlicher Frakturen hat die noch offenen Wachstumsfragen, die jeweils dem Alter entsprechend schnellere Knochenbruchheilung und eine mehr oder weniger starke Stimulierung des Längenwachstums zu berücksichtigen. Verbleibende Fragmentverschiebungen werden später um so vollständiger ausgeglichen, je jünger das verletzte Kind ist. Darstellung des konservativen Vorgehens bei Schaft-, metaphysären und Gelenkfrakturen. Sekundärschäden sind in der Regel nicht zu erwarten, daher erübrigen sich gezielte krankengymnastisch-physikalische Maßnahmen.
    Notizen: Summary The preliminary conservative management of juvenile fractures by closed reduction must be performed with due regard for the pressure of epiphyseal plates, the more rapid bone healing and the increased stimulation of growth. Malunited fractures are realigned by the normal process of bone remodeling, and results are better in young children. The management of shaft, metaphyseal and joint fractures by closed reduction is outlined. Late sequelae such as a diminished range of motion of the affected limb are rare, so that intensive physical therapy is not required.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01267383
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