ISSN:
0570-0833
Keywords:
Thin layer chromatography
;
Nucleotides
;
Chemistry
;
General Chemistry
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Thin-layer chromatography of nucleotides on DEAE- and ECTEOLA-cellulose is superior to the methods of seperation in use up to the present. Using dilute hydrochloric acid, and without a gradient elution technique, it is possinle to acheive a group seperation of monodi-, and tri-phosphates as well as subfractionation within these groups depending on the basicity of the nucleobases. Thin-layer chromatography is appreciably more sensitive than paper chromatography; 5×10-4 mole adenine neuclotides can be still be detected. The total quantity of material that can be chromatographed without tailing is greater in this procedure than that with paper chromatography; a mixture consisting of 1 μmole ATP and ADP per starting spot can be resolved with ease . Hence the procedure can also be applied for micro-scale preparative seperations . A mixtures of 10-2 μmole ADP and ATP, respectively, can be seperated completely in four to ten minutes.
Additional Material:
7 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/anie.196204351
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