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  • 1965-1969  (117)
  • 1890-1899
  • 1967  (58)
  • 1965  (59)
  • Biochemistry and Biotechnology  (117)
  • 101
    Electronic Resource
    Electronic Resource
    Die kontinuierliche Züchtung von Mikroorganismen als apparatives problem (1965)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 101-128 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Bei der kontinuierlichen Züchtung von Mikroorganismen ist die zuverlässige Aufrechterhaltung des stationären Zustandes die Grundbedingung für die erfolgreiche Untersuchung der biologischen Probleme. Auftretende Instabilitäten können apparativen oder biologischen Ursprunges sein; eine einwanddfrei funktionierende Ausrüstung ist daher bei der Einführung der Kontinuität primäres Erfordernis. Es wird auf die wichtigsten Ursachen, welche zu Instabilitäten führen können, eingegangen und eine Laborapparatur beschrieben, welche eine Basisausrüstung umfasst, die speziell für hohe Betriebssicherheit und Einfachheit der Bedienung ausgelegt wurde. Besondere Aufmerksamkeit wurde der Konstanz des Zuflusses f des Mediums, und des arbeitenden Volumens v, sowie der sicheren Ausschaltung von Infektionen durch Verwendung sterilisierbarer pH-Elektroden und eines Membranverschlusses geschenkt.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260070121
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  • 102
    Electronic Resource
    Electronic Resource
    Oxidation-reduction potential and pH patterns obtained in growth of earle's strain L cells in instrumented roux bottles (1965)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 555-558 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260070409
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  • 103
    Electronic Resource
    Electronic Resource
    Hydrocarbons (1965)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 59-60 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260070112
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  • 104
    Electronic Resource
    Electronic Resource
    Resistance of microorganisms to hydrostatic pressure (1965)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 139-159 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A small fraction, about 0.05%, of the microorganisms in milk are resistant to pressure to at least 150,000 p.s.i. The pressure-resistant microorganisms were shown to be mainly bacterial spores. Some of the pressure-resistant organisms were identified. The lethal effect of pressure is more pronounced in the solid phase than in the liquid phase of water. B. subtilis was found to be capable of surviving solid-phase transitions from Ice II, III, and V to Ice I. The lethal effect of high pressure in the liquid phase of water was found not to be counteracted by an increase in temperature. The pressure resistance of B. subtilis was found to be maximum around neutral or slightly basic pH. The damaging effect of low and high pH are counteracted by sodium chloride and glucose. It is suggested that the primary mechanism of protein denaturation by pressures above 30,000 p.s.i. is ionization and formation of ionic bonds between charged groups on proteins, altering their solubility.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260070124
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  • 105
    Electronic Resource
    Electronic Resource
    Steam corrosion and steam corrosion inhibition in autoclave sterilization of dental and surgical steel materials (1965)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 177-198 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Steam corrosion and steam corrosion inhibition of dental and surgical instrument materials during autoclave sterilization have been investigated. The corrosion tests were performed on carbon steel test samples and on round dental burrs. Cyclohexylamine or decylamine were used as corrosion inhibitors, added to the “feed water,” i.e., deionized water, to a concentration of 0.1%. Before the corrosion experiment the surfaces of the test materials were standardized and calibrated by means of ultrasonic treatment. After the experiment the corrosion products were removed from the test samples or burrs using the same ultrasonic cleaning. Deionized water was used as cleaning liquid, and was then analyzed for the amount of Fe dissolved. The amount of corrosion was expressed as μg. Fe/cm.2 sample surface area or μg. Fe/burr. The results show that considerable corrosion occurs when carbon steels are exposed to steam during autoclaving, but if cyclohexylamine or decylamine is added to the steam under the otherwise unchanged experimental conditions the total corrosion was reduced by 96-99%. No cytotoxic effect on HeLa cells could be detected with surface products possibly containing traces of amine and/or amine salts - nor with corrosion products alone - when these products were removed from the test materials by means of ultrasonic cleaning.
    Additional Material: 16 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260070127
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  • 106
    Electronic Resource
    Electronic Resource
    Production of Bacillus cereus exopenicillinase on a pilot-plant scale (1965)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 517-528 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A substrain of Bacillus cereus 569/H produced above 10,000 units of penicillinase/ ml. when grown in a pilot-plant fermentor using a medium containing Casamino acids techn. (Difco) or N-Z-Amine type B (Sheffield), and salts. Simplified purification and concentration procedures give an overall yield of 50-65% enzyme. The freeze-dried enzyme preparation had a good storage stability in vacuumsealed ampules kept at 4, 30, and 37°C. In vials containing air in the head space, partial inactivation occurred in two months at 30 and 37°C. The freezedried preparation showed satisfactory performance in the production of yoghurt fermented milk.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260070407
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  • 107
    Electronic Resource
    Electronic Resource
    Steroids and related compounds (1965)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 76-85 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260070118
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  • 108
    Electronic Resource
    Electronic Resource
    Vitamins (1965)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 85-90 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260070119
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  • 109
    Electronic Resource
    Electronic Resource
    An automatic aeration control system for biosynthetic processes (1965)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 91-100 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An automatic aeration controller was constructed to assure the optimal amount of oxygen for aerobic biosynthetic processes. A platinum electrode and a KCl bridge for the calomel electrode were inserted hermetically into the fermentor and were sterilized with the culture medium. The electrode potential developed at the surface of the Pt electrode continuously indicated the actual oxygen concentration of the medium. This served as the controlled variable of the system. The concentration of dissolved oxygen resulted from the actual demand and supply of the microorganism. The electrode potential, which was the measured variable of the process, operated a closed-loop automatic aeration system. The controller activated an air inlet valve in order to balance the controlled variable. The automatic controller operated in fermentors of industrial scale for long periods in a satisfactory manner.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260070120
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  • 110
    Electronic Resource
    Electronic Resource
    Preparation of amphotericin B-14C (1965)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 133-137 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: In a study of fermentation methods of producing amphotericin B-14C, a variety of C-14 labeled precursors were added to cultures of the amphotericin-B producing streptomycete growing in a glucose-cottonseed meal medium. Acetate-14C and propionate-14C were efficiently incorporated into the amphotericin-B while formate-14C, glucose-U-14C and methionine-14CH3 were not.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260070123
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  • 111
    Electronic Resource
    Electronic Resource
    Masthead (1965)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965) 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260070201
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  • 112
    Electronic Resource
    Electronic Resource
    Kinetic studies of gluconic acid fermentations (1965)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 229-243 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The kinetics of the gluconic acid fermentation have been studied at pH 5.8 Pseudomonas ovalis B-1486 was used as the fermenting organism. D-Gluconolactone was found as an intermediate in the fermentation and accumulated at times in large amounts. When this occurred, hydrolysis of the lactone was found to be the rate limiting reaction. This can be, under certain conditions, a poor fermentation in which to study oxygen transfer limiting conditions. Further, it is impossible to relate batch and continuous kinetic data for this fermentation unless the lactone hydrolysis reaction is taken into account.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260070204
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  • 113
    Electronic Resource
    Electronic Resource
    Oil cake meal for preparation of protein hydrolysate (1965)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 285-293 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: With a view to preparing substitutes for liver or other animal protein hydrolysates used in the treatment of protein malnutrition, a process has been developed based on sesame and mustard cakes as the starting materials. The laboratory process has been successfully scaled up to pilot plant production to compute tentative costing schedule. Essentially the process consists in defatting the cake, isolating the protein by peptization and isoelectric precipitation, enzymatically digesting the protein isolate by papain, and concentrating the digest under reduced pressure. The final product is a light brown fluffy powder, rich in almost all the essential amino acids. Growth experiments with rats have indicated that the product is comparable to commercial casein although supplementation with lysine could further enhance its biological value.
    Additional Material: 8 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260070208
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  • 114
    Electronic Resource
    Electronic Resource
    The motion of algae in turbulent flow (1965)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 295-308 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Large cultures of microscopic, unicellular algae have been proposed as a means of maintaining a life-supporting atmosphere in a closed, manned system. To achieve vigorous growth of the algal culture it is necessary to subject individual algae alternately to short periods of high intensity light and darkness. One of the means suggested for obtaining a favorable light-dark sequence for photosynthesis is to cause turbulence in a closed channel on which light is incident. Since light is rapidly attenuated in a dense suspension, there will be illuminated regions adjacent to the channel walls and a dark central core. The random motions of turbulence normal to the direction of flow would move the algae alternately from the illuminated regions to the dark region and back again. This paper indicates a method for analyzing the motion of algae into and out of the illuminated region of a channel formed by flat, parallel, transparent plates, with light incident on the plates. Matching of a probability model with a diffusion model makes it possible to estimate the light-dark sequence which could be achieved by turbulence. The results indicate that favorable sequences by this mechanism are unlikely.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260070209
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  • 115
    Electronic Resource
    Electronic Resource
    An 8-liter continuous flow culture apparatus for laboratory production of microorganisms (1965)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 367-386 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The construction of a “Porton type” chemostat of 8-l. working volume, based on the New Brunswick Scientific Co., Inc., Model F-14 Fermentor, is detailed. Instrumentation for temperature, pH, and foam control, and for medium supply are described and operational problems discussed.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260070306
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  • 116
    Electronic Resource
    Electronic Resource
    Aerobic hydrocarbon fermentation - a thermodynamic evaluation (1965)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 445-446 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260070311
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  • 117
    Electronic Resource
    Electronic Resource
    Submerged culture of hamster kidney cells in a stainless steel vessel (1965)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 417-434 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: This paper describes the design and operation of an industrial type culture system for the submerged growth of BHK 21 cells at a volume of 30 l. Using a modified Eagle's medium and with the culture controlled at pH 7.4 ± 0.05 and at a temperature of 35 ± 0.25°C., the cells grew from 0.5 × 106 to about 2.5 × 106 viable cells/ml. in 50 hr. at stirrer speeds of 330 and 460 rpm. The significance of certain aspects of design and operation of a culture system of this type is discussed.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260070309
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