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  • 2000-2004
  • 1970-1974  (2)
  • 1890-1899
  • 1810-1819
  • 1972  (2)
  • Immunohistochemistry
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 132 (1972), S. 1-14 
    ISSN: 1432-0878
    Keywords: Pituitary ; Rat ; Pars intermedia ; Adrenocorticotropin ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Adrenocorticotropin (ACTH) was localized in the intermediate lobes of normal rat pituitaries with unlabeled antibody and the soluble peroxidase-antiperoxidase complex at the electron microscopic level. All of the intermediate lobe cells (light and dark cells) stained positively for ACTH. Stain of varying intensity was found on secretory granules and vesicles. Structures which resembled rough endoplasmic reticulum stained where the secretory granules and vesicles were most numerous. No stain was on mitochondria, in nuclei, in cisternae and granules of Golgi complexes, or in rough endoplasmic reticulum where there were few secretory granules. There were distinct immunohistochemical differences between the pars intermedia and anterior lobe ACTH cells. In the ACTH cells of the anterior lobe, the Golgi complex and its granules stained strongly, but not at all in the pars intermedia cells. This suggests that ACTH may not be packaged or condensed in the Golgi region of the latter.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0878
    Keywords: Pituitary gland ; Amphibians ; ACTH ; MSH ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Les antisérums préparés à l'égard de trois hormones de synthèse [β(1–24) corticotropine, α et β MSH] nous ont servi pour localiser par immunofluorescence dans l'adénohypophyse des Amphibiens les sites d'élaboration de l'ACTH, de l'α et de la β MSH. Les anticorps anti-α et anti-β MSH se fixent exclusivement sur les cellules de lapars intermedia tandis que l'anti β-(1–24) corticotropine révèle simultanément une catégorie de cellules de lapars distalis et des cellules de lapars intermedia. D'après le contrôle cytologique effectué par la technique au bleu Alcian PAS, l'ensemble de ces cellules sont PAS positives et bleu alcian négatives. Elles réagissent également à la paraldéhyde fuchsine en l'absence d'oxydation permanganique. La signification de ces résultats est discutée.
    Notes: Summary Antisera prepared against synthetic β(1–24)corticotropin (synacthen) α and β MSH were used to localize, by immunofluorescent procedure in the adenohypophysis of Amphibians, the site of production of ACTH, α and β MSH. Anti-α and anti-β MSH antibodies appeared to be bound only to thepars intermedia cells whereas anti-β(1–24) corticotropin reacted simultaneously with specific cells of thepars distalis and cells of thepars intermedia. Cytological control by alcian blue (pH 3) PAS technique proved that all these cells are PAS positive and alcian blue negative. They react also with paraldehyde fuchsine without oxidation. The possible significance of these results is discussed.
    Type of Medium: Electronic Resource
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