ISSN:
0091-7419
Schlagwort(e):
glycoconjugate
;
glycosaminoglycan
;
hyaluronic acid
;
transformation
;
tumorigenicity
;
Life Sciences
;
Molecular Cell Biology
Quelle:
Wiley InterScience Backfile Collection 1832-2000
Thema:
Biologie
,
Chemie und Pharmazie
,
Medizin
Notizen:
Analysis of glucosamine labeled glycoconjugates in cultured cells has been made comparing 2 clones and the parent embryonic mouse cell line. Hyaluronic acid, heparan sulphate, and chondroitin sulphate as well as a complex mixture of glycopeptides were found in the medium, the trypsinate, and the trypsinized cells, although the distribution was not uniform. The 3 cell lines had very similar in vitro growth properties, including their plating efficiency in viscous medium. However, the tumorigenicity of the cells, determined in syngeneic mice, was found to differ. All 3 cell lines were found to have similar glycoconjugate distributions, although a slight relative increase in labeled hyaluronic acid was found in the more tumorigenic mass cell line than either of the clones. The possible significance of this increase is discussed.
Zusätzliches Material:
3 Ill.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1002/jss.400070109
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