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  • 1
    Electronic Resource
    Electronic Resource
    Reversion ofLactobacillus lactis protoplasts (1988)
    Springer
    Cellular and molecular life sciences 44 (1988), S. 348-351 
    Details
    ISSN: 1420-9071
    Keywords: L. lactis ; protoplasts ; protoplast regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary More than 99% ofL. lactis cells have been converted to protoplasts upon digestion of cell walls with mutanolysin (N-(acetyl)muramidase). Functional protoplasts were obtained even with the lowest level of the enzyme that was used (0.1 U·ml−1 of the cell suspension) and after incubation at 37°C for 2 min. The regeneration of the polymerized cell wall appears to be induced by a cell homogenate of the same organism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01961279
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Effects of a Ficoll-agarose system on early division and development of mesophyll protoplasts of winter oilseed rape (Brassica napus L.) (1988)
    Springer
    Plant cell, tissue and organ culture 12 (1988), S. 285-290 
    Details
    ISSN: 1573-5044
    Keywords: Brassica napus ; protoplasts ; Ficoll ; agarose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A method is described for regenerating callus from mesophyll protoplasts of a winter variety of Brassica napus. The method combines the use of Ficoll in an initial liquid medium, enhancing early protoplast division and cell colony formation, with a transfer to an agarose system after 10 days culture to give rapid microcalli formation. Further transfers resulted in callus regeneration and the initiation of organogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00034370
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Increased totipotency of protoplasts from Brassica oleracea plants previously regenerated in tissue culture (1988)
    Springer
    Plant cell, tissue and organ culture 14 (1988), S. 15-24 
    Details
    ISSN: 1573-5044
    Keywords: Brassica ; genetics of regeneration ; protoplasts ; totipotency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Factors affecting the division of cells derived from leaf and cotyledon protoplasts from Brassica oleracea L. var. italica (‘Green Comet’ hybrid broccoli) were examined to optimize conditions for plant regeneration and to determine whether there was a genetic basis for improved regeneration from protoplasts derived from plants previously regenerated from tissue cultures [15]. When leaf protoplasts from different plants grown from hybrid seed were isolated and cultured simultaneously, division efficiencies of 1–95% were obtained. Cells from some plants showed high division efficiencies in consecutive experiments while cells from other plants had consistently low division rates. More plants from hybrid seed gave high division efficiencies when cotyledon protoplasts were used. However, cotyledon or leaf protoplasts from selfed progeny of regenerated plants produced more vigorous calli and more shoots than protoplasts from hybrid seed. These results suggest that there may be a genetic component to the increased totipotency of Brassica oleracea protoplasts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00029571
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    Paper (German National Licenses)
    Fulltext
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