ISSN:
1432-0428
Keywords:
Spontaneous diabetes
;
BB rat
;
insulin
;
Δ6 desaturase
;
Δ5 desaturase
;
liver lipids
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Rat liver microsomal Δ6 and Δ5 desaturation are defective in experimental diabetes, but this defect is correctable with insulin treatment. Rat liver fatty acid composition and Δ6 and Δ5 desaturation were studied in the spontaneously diabetic adult female Bio-Breeding (BB) rat. Control Wistar rats and BB rats (4 weeks of diabetes), that received insulin (1 IU·100 g body weight−1·day−1), were killed 20 h after the last insulin injection. Δ6 and Δ5 desaturase activities were estimated from the incubation of liver microsomes with (1-14C) 18:2, n−6 or (2-14C) 20:3, n−6, respectively, and the fatty acid composition of the liver and microsomal liver lipids were investigated. Under experimental conditions Δ6 and Δ5 desaturase activities were unchanged in the BB rats when compared to the control rats. Impairment of the liver fatty acid composition of diabetic BB rats is not consistent with normal desaturase activity and may be explained by factors other than desaturation disturbance.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00264908
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