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  • 2010-2014
  • 1995-1999  (7)
  • 1955-1959
  • 1880-1889
  • 1998  (7)
  • particle bombardment
  • 1
    Electronic Resource
    Electronic Resource
    An Efficient Rice Transformation System Utilizing Mature Seed-derived Explants and a Portable, Inexpensive Particle Bombardment Device (1998)
    Springer
    Transgenic research 7 (1998), S. 289-294 
    Details
    ISSN: 1573-9368
    Keywords: Oryza sativa ; particle bombardment ; transformation ; transgenic rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We developed a practical and efficient gene transfer system for indica rice utilizing mature-seed derived explants and a simple bombardment device which uses compressed helium for accelerating DNA-coated metal particles. Unlike instruments which have been described in the literature previously, this new bombardment device, which is an improvement of the particle inflow concept, does not require vacuum. This attribute simplifies the transformation procedure significantly and it makes rice transformation technology accessible to laboratories which may not have the resources to invest in more expensive particle bombardment instruments. We determined experimentally that we could recover transgenic rice plants utilizing three different particle bombardment instruments at comparable frequencies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008870012568
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Molecular Characteristics of Transgenic Wheat and the Effect on Transgene Expression (1998)
    Springer
    Transgenic research 7 (1998), S. 463-471 
    Details
    ISSN: 1573-9368
    Keywords: particle bombardment ; transgene expression ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A population of R0 transgenic wheat plants, generated by particle bombardment, was analyzed to define molecular, genetic and phenotypic properties resulting from transformation with a cointegrate vector, or cotransformation with two separate plasmids. By evaluating the progeny of 70 independently-derived transgenic plants, we also identified rare events such as chimerism and transgene elimination, which provide valuable information concerning the development of transgenic cereal plants following bombardment experiments. The frequency of chimerism in our transgenic wheat plants was very low. Furthermore, while transgene elimination did occur, this was also a very rare event. We determined the copy numbers of integrated transgenes and the levels of transgene expression. Comparisons to transgenic rice plants generated in the same manner demonstrated some similarities, but also important differences in transgene behavior. Whereas in rice there is no evidence for any direct relationship between transgene copy number and transgene expression or stability, multicopy populations in wheat demonstrated a bias towards higher levels of expression for the two genes and the maize ubiquitin promoter evaluated in the present study.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008833324193
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Molecular analysis of the genome of transgenic rice (Oryza sativa L.) plants produced via particle bombardment or intact cell electroporation (1998)
    Springer
    Molecular breeding 4 (1998), S. 99-109 
    Details
    ISSN: 1572-9788
    Keywords: transgenic rice ; particle bombardment ; cell electroporation ; RAPD ; AFLP ; AFRP ; RAMP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In the present work we utilised some of the most discriminative molecular tools, such as RAPD, AFLP, AFRP and RAMP, to analyse the genome of independently derived transgenic plants from three elite Italian cultivars (cv. Lido, Carnaroli and Thaibonnet) and found that two methods for direct gene transfer, namely particle bombardment and intact cell electroporation (the latter being a procedure set up in this work), result in transgenic rice (Oryza sativa L.) plants that exhibit negligible genomic changes. This is in contrast with recently published results showing relevant changes in the DNA of transgenic rice plants generated through protoplasts electroporation and of transgenic poplar plants engineered through Agrobacterium tumefaciens infection. Implications of these findings are discussed in the context of selecting appropriate gene transfer methodologies to produce transgenic plants expressing genes of interest while retaining their genomic integrity and, thus, their superior agronomic and/or industrial traits.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009627409668
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Effective control of yellow stem borer and rice leaf folder in transgenic rice indica varieties Basmati 370 and M 7 using the novel δ-endotoxin cry2A Bacillus thuringiensis gene (1998)
    Springer
    Molecular breeding 4 (1998), S. 501-507 
    Details
    ISSN: 1572-9788
    Keywords: Bacillus thuringiensis (Bt) ; cry2A ; particle bombardment ; rice leaf folder ; transgenic rice ; yellow stem borer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Transgenic rice indica varieties Basmati 370 and M 7 expressing the novel cry2A (Bt) insecticidal gene were generated by particle bombardment. Molecular and biochemical analyses in R0 and R1 populations confirmed stable integration and expression of this novel Bt transgene. We estimated that the gene product was expressed up to 5% of total leaf protein. Insect feeding bioassays demonstrated that the Cry2A protein was effective against the yellow stem borer and the rice leaf folder, two major rice pests in the Indian Subcontinent. This is the first report of the control of major rice pests using this specific Bt gene. The cry2A gene can now be used in combination with other insecticidal genes for pyramiding resistance against insect pests. This will delay, or perhaps in combination with integrated pest management practices, prevent evolution of insect populations resistant to single insecticidal genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009660315970
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Transgenic beans (Phaseolus vulgaris L.) engineered to express viral antisense RNAs show delayed and attenuated symptoms to bean golden mosaic geminivirus (1998)
    Springer
    Molecular breeding 4 (1998), S. 491-499 
    Details
    ISSN: 1572-9788
    Keywords: BGMV ; common bean ; particle bombardment ; plant transformation ; virus resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The genes Rep-TrAP-REn and BC1 from the Brazilian isolate bean golden mosaic geminivirus (BGMV-BR) were cloned in antisense orientation under the transcriptional control of the CaMV 35S promoter. This construct was used to transform common bean (Phaseolus vulgaris L.) using the biolistic method. Transgenic plants from the R3 and R4 generations were challenged by inoculation with a BGMV-BR viruliferous whitefly population. Of the four transgenic lines tested, two had both delayed and attenuated viral symptoms. Un-transformed plants or plants transformed with a construct containing only the gus-neo gene developed typical BGMV-BR symptoms 10–15 days after inoculation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009613607559
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Tissue-specificity of maize sucrose synthase gene promoters in maize tissues after particle bombardment (1998)
    Springer
    Euphytica 103 (1998), S. 17-21 
    Details
    ISSN: 1573-5060
    Keywords: particle bombardment ; promoter ; tissue-specificity sucrose synthase ; transient expression ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The reporter gene encoding β-glucuronidase (GUS) driven by either of the two maize sucrose synthase gene (Sh1 and Sus 1) promoters was introduced and expressed in various maize tissues via particle bombardment. Transient gene expression was examined by histochemical assays. It was found that the two SS promoters directed differential GUS expression. In the developing kernel, the Sh1 promoter was active only in the upper and central parts of the endosperm. In contrast, strong GUS activity controlled by the Sus1 promoter was detected in various types of cells, including the aleurone cells, the subaleurone endosperm cells, the scutellar cells of the embryo and the pericarp cells. Both promoters showed similar expression patterns in vegetative tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018326915934
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Expression and Immunolocalisation of the Snowdrop Lectin, GNA in Transgenic Rice Plants (1998)
    Springer
    Transgenic research 7 (1998), S. 371-378 
    Details
    ISSN: 1573-9368
    Keywords: rice transformation ; GNA ; particle bombardment ; immunolocalisation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transgenic rice (Oryza sativa L.) plants generated through particle bombardment expressed high levels of an insecticidal protein (the snowdrop lectin, GNA) directed against sap-sucking insects. Engineered plants expressed GNA either constitutively or in a tissue specific manner, depending on the nature of the promoter used to drive expression of the gene. We used specific antibodies raised against GNA to localize its expression in phloem tissue in plants engineered with the rice sucrose synthase promoter driving GNA expression. We report here molecular, biochemical and immunological analyses for fifteen independently-derived transformants out of more than 200 plants we generated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008856703464
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    Paper (German National Licenses)
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