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  • 1975-1979  (10)
  • 1955-1959  (7)
  • 1880-1889
  • 1830-1839
  • 1800-1809
  • Brassica napus
  • gene expression
  • 1
    Electronic Resource
    Electronic Resource
    The possibility of predicting solute uptake and plant growth response from independently measured soil and plant characteristics (1979)
    Springer
    Plant and soil 53 (1979), S. 169-191 
    Details
    ISSN: 1573-5036
    Keywords: Brassica napus ; Growth analysis ; NO3 uptake rates ; Sensitivity analysis ; Soil solution ; Uptake simulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Rape plants were grown in a constant environment for 24 days in a sandy loam soil initially at soil solution nitrate concentrations of 1.54×10−2 M and 3.72×10−3 M. Nutrients other than nitrate were maintained at adequate levels. The growth and nitrate uptake were followed by successive harvests of replicate plants. The dry weights and N contents of plants were compared with those predicted by the model in Paper I of this series8, based on parameters of solution cultured plants. Similar environmental conditions were reproduced for these soil culture experiments and soil characteristics were measured separately. The predicted and observed N contents agreed well over the entire growth period. The dry weights were predicted accurately for 15 days in both cases, but were overestimated when extrapolated beyond the range of the model. The dry weight and N content were most sensitive to the relative growth rate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02181889
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  • 2
    Electronic Resource
    Electronic Resource
    Transfer of resistance to race 2 of Plasmodiophora brassicae from Brassica napus to cabbage (B. oleracea ssp. capitata). III. First backcross and F2 progenies from interspecific hybrids between B. napus and B. oleracea ssp. capitata (1979)
    Springer
    Euphytica 28 (1979), S. 257-266 
    Details
    ISSN: 1573-5060
    Keywords: Crucifers ; Brassica napus ; Brassica oleracea ssp. capitaca ; cabbage ; Plasmodiophora brassicae ; clubroot disease ; fungal resistance ; interspecific cross ; resistance breeding ; chromosome numbers ; meiosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The first backcross and F2 progenies from triploid F1 and tetraploid F1 hybrids between B. napus and 2x and 4x B. oleracea ssp. capitata (cabbage) were studied for their general morphology, resistance to race 2 of the clubroot pathogen, chromosome number and meiotic chromosome behavior. No linkage was apparent between resistance and the major morphological characters. Unreduced gametes played a large part in the successful formation of seed of the B1 and F2 progeny. B1 plants with low chromosome numbers were selected for use in recurrent backcrosses. The potential use of anther culture to extract gametic progenies from resistant B1 and F2 plants with higher chromosome numbers was suggested. The presence of homoeologous pairing observed in all the plants is considered advantageous for selecting suitable progeny in later generations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00056583
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  • 3
    Electronic Resource
    Electronic Resource
    The possibility of predicting solute uptake and plant growth response from independently measured soil and plant characteristics (1979)
    Springer
    Plant and soil 53 (1979), S. 137-167 
    Details
    ISSN: 1573-5036
    Keywords: Brassica napus ; Growth analysis ; NO3 uptake rates ; Ontogenetic effects ; Root demand coefficient ; Root hairs ; Solution culture ; Shoot: root ratios ; Tissue N concentration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The growth and nitrogen uptake response of rape plants to nitrate concentration at the root surface were studied in solution culture in a controlled environment cabinet over a period of 24 days. NO3 − was supplied at the rates of 10−5 M, 5×10−5 M, 10−4 M, 10−3 M and 10−2 M in solution and was maintained near these levels. With increasing mean N concentration in the tissues, the relative growth rate and leaf area ratio increased and unit leaf rate decreased slightly. Values of all three growth parameters decreased with plant age. The shoot: root dry weight ratios and their N content ratios increased with increasing mean per cent N in the plant. The length or surface area per unit dry weight of roots was correlated negatively with per cent N and positively with age. The maximum mean inflow of nitrate to rape roots decreased sharply with age. The concentration at which half maximal mean inflow was attained was 3.44×10−5 M NO3 −.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02181888
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  • 4
    Electronic Resource
    Electronic Resource
    The possibility of predicting solute uptake and plant growth response from independently measured soil and plant characteristics (1979)
    Springer
    Plant and soil 53 (1979), S. 193-201 
    Details
    ISSN: 1573-5036
    Keywords: Brassica napus ; Growth analysis ; NO3 uptake rates ; Root length ratio ; Solutionversus soil solution ; Uptake analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The growth and nitrate uptake characteristics of rape plants grown in soil at initial NO3 − concentrations of 1.54×10−2 M and 3.72×10−3 M are compared with those observed in solution culture at 10−2 M and 10−3 M. The relative growth rates and total nitrogen concentration of the soil grown plants were similar to those observed in solution culture. This suggests a remarkable capacity of the plant to regulate the size and efficiency of the photosynthetic and uptake machineries. Variations in the unit leaf rate or nitrate inflow in response to the environment or the internal nutrient status are counterbalanced by variations in the leaf area ratio or in ‘root length ratio’ so as to maintain a similar course of growth. The shoot: root ratios and the root length per unit dry weight varied within similar ranges in soil and solution. The soil grown plants accumulated N preferentially in shoots compared with those grown in solution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02181890
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  • 5
    Electronic Resource
    Electronic Resource
    Transfer of resistance to race 2 of Plasmodiophora brassicae from Brassica napus to cabbage (B. oleracea var. capitata). II. Meiosis in the interspecific hybrids between B. napus and 2x and 4x cabbage (1978)
    Springer
    Euphytica 27 (1978), S. 81-93 
    Details
    ISSN: 1573-5060
    Keywords: Brassica oleracea var. capitata ; cabbage ; Brassica napus ; rape ; colza ; crucifers ; Plasmodiophora brassicae ; clubroot disease ; fungal resistance hybrids ; meiosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Meiosis in 14 interspecific F1 hybrids with three chromosomal levels (triploid, tetraploid, hexaploid; 2n=28, 37 and 55) between Brassica napus L. and 2x and 4x cabbage (B. oleracea var. capitata L.) was studied. The oleracea genome from B. napus maintained close homology with the c genome of cabbage while the campestris genome of B. napus showed partial homology with the c genome contained in the hybrids. Genotypic influence on chromosome pairing was indicated. Structural chromosome differences and spontaneous chromosome breakage and reunion were suggested as causes for the abnormalities which related to the unbalance of the genotypes. The divergence of the genomes of B. napus and B. oleracea and the need for the qualification of the term secondary association were discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039122
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  • 6
    Electronic Resource
    Electronic Resource
    A genetic study of growth characters and yield characters of oilseed rape (1978)
    Springer
    Euphytica 27 (1978), S. 177-183 
    Details
    ISSN: 1573-5060
    Keywords: Brassica napus ; oilseed rape ; yield ; yield components ; growth pattern ; inheritance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Analysis of single plants in parental, F1 and F2 populations of three crosses of oilseed rape indicated cytoplasmic effects in F1 or F2 generations for most of the observed characters. There was evidence of dominance for early flowering and early maturity. Heterosis was exhibited for yield and yield components. The heritabilities for the characters under study were generally very low.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039133
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  • 7
    Electronic Resource
    Electronic Resource
    A study on disease variation in the populations of an interspecific cross of Brassica juncea L. x B. napus L. (1978)
    Springer
    Euphytica 27 (1978), S. 145-149 
    Details
    ISSN: 1573-5060
    Keywords: Brassica juncea ; Brassica napus ; interspecific cross ; seedling/adult resistance ; diseases of rapes ; Leptosphaeria maculans ; blackleg
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary F1 behaviour and F2 variation in disease reaction were studied in the interspecific cross Brassica juncea x B. napus. Gene(s) for adult resistance to blackleg (Leptosphaeria maculans) were found to be present in the A genome of B. juncea and could be transferred to B. napus. Gene(s) for complete (seedling plus adult) resistance in B. juncea appeared to be located in the B genome. The chance of their transfer to the oilseed rapes (B. napus or B. campestris) would therefore seem to be remote.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039129
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  • 8
    Electronic Resource
    Electronic Resource
    The introgression of S alleles into forage rape, Brassica napus L. from turnip. Brassica campestris L. ssp. Rapifera (1977)
    Springer
    Euphytica 26 (1977), S. 511-519 
    Details
    ISSN: 1573-5060
    Keywords: Brassica napus ; rape ; Brassica campestris ; turnip ; introgression ; S incompatibility alleles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Forage rape. B. napus, is self-compatible, the work described illustrates the introgression of functional incompatibility alleles into B. napus from turnips, B. campestris, in two generations. By grading seed, produced by backcrossing turnip/rape hybrids to rape, the frequency of 2n=38 ‘semi articial’ B. napus recovered, amply justifies the cytological screening involved in such breeding programmes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00027020
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  • 9
    Electronic Resource
    Electronic Resource
    Transfer of resistance to race 2 of Plasmodiophora Brassicae from Brassica napus to cabbage (B. oleracea var. Capitata). I. Interspecific hybridization between B. napus and B. oleracea var. Capitata (1977)
    Springer
    Euphytica 26 (1977), S. 319-336 
    Details
    ISSN: 1573-5060
    Keywords: Crucifers ; Brassica napus ; Brassica oleracea ssp. capitata ; cabbage ; Plasmodiophora brassicae ; clubroot disease ; fungal resistance ; interspecific cross ; resistance breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Interspecific hybridization between Brassica napus L. (2n=38, a1a1c1c1) and B. oleracea var. capitata L. (2x- and 4x-cabbage; 2n=2x=18, cc and 2n=4x=36, cccc) was carried out for the purpose of transferring clubroot disease resistance from the amphidiploid species to cabbage. Nineteen hybrids with three different chromosome levels (2n=28, a1c1c; 2n=37, a1c1cc and 2n=55, a1c1cccc) were obtained. The F1 plants were mostly intermediate between the two parents but as the number of ‘c’ genomes in the hybrids increased, the more closely the hybrids resembled the cabbage parent. All F1 hybrids were resistant when tested against race 2 of Plasmodiophora brassicae wor. The complete dominance of resistance over susceptibility suggested that the gene(s) controlling resistance to this particular race of the clubroot pathogen is probably located on a chromosome of the ‘a’ genome in Brassica.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00026993
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  • 10
    Electronic Resource
    Electronic Resource
    Lactate dehydrogenase in preimplantation rabbit embryos (1975)
    Springer
    Biochemical genetics 13 (1975), S. 663-671 
    Details
    ISSN: 1573-4927
    Keywords: lactate dehydrogenase ; isoenzymes ; differentiation ; blastocyst ; gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Unfertilized eggs and early embryos up to the 2-day (16-cell) cleavage stage of development in the rabbit contain predominantly the most cathodal lactate dehydrogenase isoenzyme made up of A-type subunits. Following early cleavage there is a progressive increase in total LDH activity in the embryo as development proceeds through 4- and 6-day blastocyst stages. This is accompanied by an increase in the amounts of B-type subunits and a concomitant shift in the lactate dehydrogenase isoenzyme electrophoretic pattern toward the anodal isoenzyme types.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00484924
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  • 11
    Electronic Resource
    Electronic Resource
    Microprotoplast fusion technique: a new tool for gene transfer between sexually-incongruent plant species (1955)
    Springer
    Euphytica 85 (1955), S. 255-268 
    Details
    ISSN: 1573-5060
    Keywords: microprotoplast fusion ; partial genome transfer ; monosomic additions ; kanamycin resistance ; β-glucuronidase ; gene expression ; potato
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Various aspects of a microprotoplast fusion technique and the strategies followed for intergeneric partial genome transfer (one or a few chromosomes) and alien genes from sexually-incongruent donor species to recipient species are described. The essential requirements of the microprotoplast fusion technique are the induction of micronuclei at high frequencies, as well as the isolation and enrichment of sub-diploid microprotoplasts in donor species, efficient fusion of the donor microprotoplasts with normal recipient protoplasts and stable regeneration of plants from fusion products. The results on the production of microprotoplast hybrid plants between the transformed donor lines of Solanum tuberosum and Nicotiana Plumbaginifolia carrying various genetic markers, and a recipient line of Lycopersicon peruvianum or Nicotiana tabacum, and on the transfer and expression of alien genes (kanamycin resistance, β-glucuronidase) are presented. The data obtained on microprotoplast hybrid plants between S. tuberosum and L. peruvianum showed that many of the hybrids contained one potato chromosome carrying nptII and GUS, and 24 or 48 L. peruvianum chromosomes (monosomic additions), and that they were male-and female-fertile. Various applications of chromosome transfer by this technique, especially for economically-important traits (e.g. disease or stress resistance) from sexually-incompatible wild species, for construction of chromosome-specific DNA libraries through microdissection and microcloning of chromosomes, or by flow-sorting of chromosomes for genome analysis, are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023954
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  • 12
    Electronic Resource
    Electronic Resource
    A study of different (CaMV 35S and mas) promoter activities and risk assessment of field use in transgenic rapeseed plants (1955)
    Springer
    Euphytica 85 (1955), S. 411-416 
    Details
    ISSN: 1573-5060
    Keywords: Agrobacterium ; Brassica napus ; CaMV 35S promoter ; mas promoter ; gene expression ; risk assessment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Gene fusions between the β-glucuronidase (GUS) reporter gene and the promoters of the cauliflower mosaic virus 35S RNA transcript (CaMV 35S) and the mannopine synthase (mas) genes were introduced into rapeseed varieties via Agrobacterium-mediated transformation. Fluorometric assay of β-glucuronidase activity indicated different expression patterns for the two promoters. In seedlings, the CaMV 35S promoter had maximum activity in the primary roots, while the mas promoter was most active in the cotyledons. Etiolated seedlings cultured in the dark showed reduced activity of the mas promoter. Before vernalization at the rosette stage, both promoters were more active in older plant parts than in younger ones. At this stage the highest activity was recorded in cotyledons. After the plants had bolted reduced promoter function was detected in the upper parts of the transformed plants. Both promoters were found to be functional in the majority of the studied organs of transgenic rapeseed plants, but the promoter activity varied considerably between the organs at different developmental stages. The ability of pollen to transfer the introduced genes to other varieties and related species (e.g. Brassica napus and Diplotaxus muralis) by cross-pollination was studied in greenhouse experiments, and field trials were carried out to estimate the distance for biologically-relevant gene dispersal. In artificial crossing, the introduced marker gene was transferable into other varieties of Brassica napus. In field trials, at a distance of 1 metre from the source of transgenic plants, the frequency of an outcrossing event was relatively high (10-3). Resistant individuals were found at 16 and 32 metres from the transgenic pollen donors, but the frequency of an outcrossing event dropped to 10-5.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023974
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  • 13
    Electronic Resource
    Electronic Resource
    Transfer of disease resistance within the genus Brassica through asymmetric somatic hybridization (1955)
    Springer
    Euphytica 85 (1955), S. 247-253 
    Details
    ISSN: 1573-5060
    Keywords: asymmetric somatic hybridization ; Brassica napus ; Brassica nigra ; disease resistance transfer ; dot blot analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Asymmetric somatic hybrid plants between Brassica napus L. (oilseed rape genome AACC) and a transgenic line of Brassica nigra L. Koch (black mustard genome BB) were tested for their resistance against rapeseed pathogens Phoma lingam (black leg disease) and Plasmodiophora brassicae (club root disease). The transgenic B. nigra line used (hygromycin-resistant, donor) is highly resistant to both fungi, whereas B. napus (recipient) is highly susceptible. The asymmetric somatic hybrids were produced using the donor-recipient fusion method (with X-irradiation of donor protoplasts) reported by Zelcer et al. (1978) for the production of cybrids. Using hygromycin-B for selection, a total of 332 hybrid calli were obtained. Regenerants, resistant or susceptible to both diseases, were selected. Many hybrids expressed resistance to only one pathogen. Dot blot experiments showed that the asymmetric hybrid plants contained varying amounts of the donor genomic DNA. Furthermore, a correlation was detected between the radiation dose and the degree of donor DNA elimination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023953
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  • 14
    Electronic Resource
    Electronic Resource
    Degradation of oxalic acid by transgenic oilseed rape plants expressing oxalate oxidase (1955)
    Springer
    Euphytica 85 (1955), S. 169-172 
    Details
    ISSN: 1573-5060
    Keywords: Brassica napus ; disease tolerance ; oxalic acid ; oxalate oxidase ; Sclerotinia sclerotiorum ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Oxalic acid is thought to have a primary role in the pathogenicity of several plant pathogens, notably Sclerotinia selerotiorum. A gene coding for the enzyme oxalate oxidase was isolated from barley roots and introduced into oilseed rape as a means of degrading oxalic acid in vivo. This report describes the production of several transgenic plants of oilseed rape and the characterisation of these plants by Southern, Western and enzyme activity assays. Plants were shown to contain an active oxalate oxidase enzyme and were tolerant of exogenously supplied oxalic acid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023945
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  • 15
    Electronic Resource
    Electronic Resource
    Modification of oilseed rape to produce oils for industrial use by means of applied tissue culture methodology (1955)
    Springer
    Euphytica 85 (1955), S. 323-327 
    Details
    ISSN: 1573-5060
    Keywords: Brassica napus ; fatty acids ; gas chromatography ; Lunaria annua ; protoplast regeneration ; somaclonal variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A programme of research was designed to investigate methods for the modification of the fatty acid profiles of high performance lines of oilseed rape (Brassica napus L.) in an attempt to produce lines with enhanced levels of industrially useful fatty acids. The methodology employed to achieve these objectives was based on the exploitation of somaclonal or protoclonal variation, and targeted somatic hybridization using wild cruciferous germplasm as fusion partners. A range of somaclonal lines was produced from shoot regeneration protocols. These lines underwent replicated, randomised glasshouse trials for morphological assessment followed by gas chromatographic analysis to monitor any changes in fatty acid profile. It was found that a small number of lines exhibited potentially useful changes in oleic acid and polyunsaturated fatty acid content. Protoplast regeneration and electrofusion protocols for a range of winter oilseed rape lines were developed, and methods for the isolation and fusion of protoplasts of the wild crucifer Lunaria annua (chosen for its high nervonic acid content) established.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023962
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  • 16
    Electronic Resource
    Electronic Resource
    The manipulation and modification of tomato fruit ripening by expression of antisense RNA in transgenic plants (1955)
    Springer
    Euphytica 85 (1955), S. 193-202 
    Details
    ISSN: 1573-5060
    Keywords: carotenoids ; ethylene ; gene expression ; Lycopersicon esculentum Mill. ; polygalacturonase ; pectinesterase ; phytoene synthase ; ACC oxidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The common cultivated tomato (Lycopersicon esculentum Mill.) provides a major focus for improvement of crop quality through genetic engineering. Identification of ripening-related cDNAs has enabled the modification of specific aspects of ripening by manipulating gene expression in transgenic plants. By utilizing ‘antisense RNA’ to modify expression of ripening genes, we have inhibited the production of the cell wall-metabolising enzymes polygalacturonase and pectinesterase and created transgenic plants that contain, effectively, single, targeted mutations affecting these genes. Furthermore, this approach has been used with previously unidentified cDNA clones to enable both functional identification and manipulation of genes involved in ethylene production (ACC oxidase) and carotenoid biosynthesis (phytoene synthase). The use of antisense RNA targeted to specific genes to alter ripening phenotypes and improve commercial utility of fruit by affecting shelf-life, processing characteristics and nutritional content is discussed. We have used the extreme ripening-impaired mutant, ripening inhibitor (rin) to identify additional genes implicated in the ripening process. This approach has resulted in the cloning of several novel ripening-related mRNAs which are now being studied by antisense experiments. This may enable identification and manipulation of additional genes involved in processes such as softening, flavour and aroma generation and susceptibility to pathogens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023948
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  • 17
    Electronic Resource
    Electronic Resource
    The sulphur-rich Brazil nut 2S albumin is specifically formed in transgenic seeds of the grain legume Vicia narbonensis (1955)
    Springer
    Euphytica 85 (1955), S. 181-192 
    Details
    ISSN: 1573-5060
    Keywords: Vicia narbonensis ; gene transfer ; gene expression ; seeds ; 2S albumin ; methionine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Epicotyl explants were co-cultivated with Agrobacterium tumefaciens EHA101 to transfer a chimeric 2S albumin gene construct carried in the binary Ti plasmid vectors pGSGLUC1 or pGA472 into the grain legume Vicia narbonensis. This gene encoding the sulphur-rich Brazil nut albumin was under the control of either the CaMV 35S promoter which permits gene expression in all organs, or the Vicia faba legumin B4 promoter which elicits seed-specific gene expression. After callus formation and selection for kanamycin resistance, somatic embryos were induced which, in the case of transformation with the vector pGSGLUC1, were screened for GUS activity. Embryos that produced GUS were in addition analysed for 2S albumin formation. Selected transgenic embryos were cloned by multiple shoot regeneration. Rooted and fertile plants were obtained by grafting transgenic shoots on the appropriate seedlings. R1 and R2 generations were raised and analysed for GUS as well as 2S albumin gene expression. Expression of the 35S promoter/2S albumin gene fusion took place in all organs of the transgenic plants including the cotyledons of seeds, whereas seed-specific gene expression was found in transformants with the legumin promoter/2S albumin gene fusion. The 2S albumin accumulated in the 2S protein fraction of transgenic seeds and its primary translation product was processed into the 9 and 3 kDa polypeptide chains. The foreign protein was localised in the protein bodies of the grain legume. Analysis of the R2 plants indicated Mendelian inheritance of the 2S albumin gene. In homozygous V. narbonensis plants the amounts of 2S albumin were twice that present in the corresponding heterozygous plants. Whereas only low level formation of the foreign protein was achieved if the gene was under the control of the 35S promoter, approximately 3.0% of the soluble seed protein was 2S albumin if seed-specific gene expression was directed by the legumin B4 promoter. Some of these transformants exhibited a three-fold increase in the methionine content of the salt-soluble protein fraction extracted from seeds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023947
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