Library

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • 1965-1969  (2,026)
  • Life and Medical Sciences  (2,026)
  • 1
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: A quantitative electron microscopic study of the clefts of Schmidt-Lantermann in fibers of the sciatic nerves of young and adult rats showed that the clefts are formed by two conical “male” and “female” portions of the sheath. The circumference of the female cone is approximately 15% larger than the male cone. The surfaces of the cleft transect the sheath at angle of approximately 9°. The present data indicate, also, that both the configuration of the conical portions of the sheath and their position relative to each other may change prior to fixation. Calculations indicate that each cleft permits a change in axoplasmic volume by 22%, and a change in fiber length by 9% of the fiber diameter.Clefts were not observed in nerves of rats before the twelfth day of age nor in sheaths of fibers composed of less than 20 lamellae. Newly formed clefts were wider than adult clefts. Transitional forms suggest that clefts may develop from fibers with redundant loops of myelin sheath. Radioautographic data on the incorporation of H3-leucine in nerve fibers did not indicate transport of significant amounts of this isotope into the clefts.
    Additional Material: 13 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 2
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The fine structure of the extracellular matrix during epithelio-mesenchymal interactions in the rabbit embryonic tooth germ is described using light and electron microscopy. Observations are restricted to the labial cervical loop region at the bell stage of development in maxillary and mandibular incisor tooth germs. Histochemical methods demonstrate a PAS-positive basement membrane between outer enamel epithelial cells and adjacent undifferentiated mesenchymal cells. The metachromatic region circumscribes the dental papilla region, becoming more intense and wider in association with cells illustrative of more advanced stages of differentiation. Ultrastructural observations of the basal lamina associated with outer enamel epithelial cells, cervical proliferating epithelial and mesenchymal cells, and with inner enamel epithelial cells and pre-odontoblasts are reported. The characteristic mesenchymal filopodia appeared in close proximity to the basal lamina. Microfibrils are seen as depositions on the basal lamina and in association with filopodia. The concentration of intercellular microfibrils is increased when in association with epithelia showing advanced differentiation. Collagen fibrils are frequently noted in peripheral association with mesenchymal cells but not adherent to the basal lamina. Numerous homotypic and heterotypic cell junctions are seen. An understanding of intercellular communication between heterotypic cells may be enhanced by postulating a dual developmental origin for the microfibrils associated with the basal lamina. The under surface of the basal lamina facing the extended mesenchymal filopodia appears to be a significant factor during epithelio-mesenchymal interactions, subsequent extracellular matrix formation, and morphogenesis.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 3
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Young and senescent female hamsters were sacrificed on days 8, 10, 12, 14 and 15 of pregnancy, and the number of viable and resorbing conceptuses was determined. The ovulation rate for each group was also recorded. At each day, there was a reduction in the litter size in senescent animals as compared to the young, but the number of ovulations in each age group was equal. The results indicated that the litter size loss, in senescent hamsters, is not due to a reduction in the ovulation rate, but to a seven fold increase in preimplantation deaths and to a two fold increase in resorption.
    Additional Material: 4 Tab.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 4
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Ovaries from young and senescent hamsters were examined morphologically on days 8, 12 and 14 of pregnancy to determine if there were any differences between the two age groups. The senescent hamsters had fewer follicles present than the young on all days of gestation. The senescent females' corpora lutea experienced the greatest growth between days 8 to 12, whereas corpora lutea from young animals grew the most between days 12 and 14. Corpora lutea in senescent females did not grow at all between days 12 and 14. The lower number of follicles in senescent females was not due to the lower rate of implantation. Superovulation with PMS revealed that the senescent ovary was refractory.It was concluded that there are quantitative not qualitative differences between ovaries from young and senescent hamsters during pregnancy. The refractory ovary of senescent females could be contributing to pregnancy wastage in this species by secreting less progesterone.
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 5
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Female mice of a Swiss albino stock were fed a high-fat, low-protein, hypolipotropic diet which produces rapid cardiovascular lesions in this stock of mice. Half of the animals were killed after 45 days and the others were placed back on a diet of normal laboratory chow and killed after one, two and three weeks of refeeding.In animals killed after receiving the experimental diet for 45 days small, focal deposits of hyalin in media of large coronary arteries of the right ventricle was the major change.During the first week of recovery (fed a normal diet) the incidence and severity of arterial lesions increased and within individual hearts arteries of all sizes and in both atria and both ventricles were involved. Following hyalinization an acute arteritis developed that involved all layers of the arterial wall and extended into the perivascular space.After two and three weeks recovery the coronary arteries were characterized by a periarterial fibrosis and by a decrease or absence of the leucocytic reaction. A subendothelial hyperplasia of smooth muscle, arranged longitudinally, was accompanied by an increase in reticular and collagenous fibers. Circular smooth muscle in the media was also hyperplastic.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 6
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 165 (1969), S. 391-399 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Female mice of the Taconic Swiss stock were fed one of the three diets described below then injected subcutaneously with 0.035 cm3CCl4/100 gm body weight in a mineral oil solution. One group was fed an adequate commercial laboratory ration, then mice were killed 12, 24 and 48 hours after CCl4; another a cholinedeficient, high-fat, low-protein diet for 14 days, then injected with CCl4, and killed 12, 24 and 48 hours later; and another group, a choline-supplemented (2 gm choline chloride/100 gm diet), high-fat, low-protein diet for 14 days, then injected with CCl4 and killed 24 hours later.In the group fed the normal diet, the polyhalogen produced within 24 hours the expected centrolobular necrosis and glycogen depletion, involving one-third to one-half of the lobular areas. In fatty livers produced by feeding the choline-deficient diet, necrosis at 24 hours post injection was limited in a majority of instances to a zone one or two cells thick immediately surrounding the central vein. Despite this decreased necrosis in fatty livers centrolobular glycogen depletion still involved one-third to one-half of lobules and there was an infiltration of inflammatory cells immediately adjacent to the central vein. The hepatic necrosis at 12 and 48 hours paralleled that in the normal dietary group.Feeding of the choline-supplemented, high-fat, low-protein diet for two weeks produced an extremely limited parenchymal liposis. In such relatively non-fatty livers CCl4 produced hepatic necrosis comparable to that in mice receiving normal diets. The extent of protection from the necrogenic actions of CCl4 was clearly associated with extensive intracytoplasmic liposis of hepatic parenchyma.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 7
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 165 (1969), S. 411-429 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: A study was made of 174 eggs recovered from 58 guinea pigs killed between the first and sixth days after mating, and the cytological details from the stage of sperm penetration to the pre-implantation blastocyst described. Eighty-nine percent of the expected number of eggs were recovered, and 97% of these were fertilized. Embryonic development proceeded from pronuclear and 2-cell eggs on day 1 to pre-implantation blastocysts on day 6, the duration of each of the early cleavage stages being of approximately 24 hours. No progressive loss of embryos was found over this period, all the eggs recovered on days 4, 5 and 6 being regarded as developing normally. Passage of embryos from the Fallopian tubes to the uterus commenced on the afternoon of day 3, and the majority of embryos entered the uterus shortly after this time at the stage of 8- to 16-cells. The tail of the fertilizing spermatozoon was detected in the cytoplasm of all eggs examined on day 1, in most of those examined on day 2, and frequently persisted up to the 8-cell stage. Three dispermic eggs, each containing two distinct cytoplasmic sperm tails, were found: one of these was at the pronuclear stage, the other two being considered of 4- and 6-cells respectively. Properties of adhesiveness were shown by blastocysts recovered shortly before implantation on day 6, but the characteristic “zona processes” were not seen.
    Additional Material: 7 Tab.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 8
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 165 (1969), S. 453-465 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The ultrastructural aspects of the chick embryo primitive erythrocytic cell series are described. There are two points of particular interest: the appearance of ferritin in erythroblast cytoplasm in 20 somite stage embryos and the behaviour of ribosomes during the red cell maturation period, both with regard to their progressive diminution and how they are grouped in polyribosomes. Ribosomes are still present in primitive series erythrocytes, although fewer than in the immature cells, and they are mainly in group form.A morphological comparison of the primitive red series erythrocytes with those of the definitive red series has also been made.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 9
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Histological and ultrastructural studies on the medial meniscus in the human knee joint show that nerve fibres, both myelinated and unmyelinated, extend from the periarticular plexus into the meniscus as far as the intermediate third. These neural elements are not exclusively paravascular in position and it is reasonable to postulate a function other than vasomotor or vasosensory for them.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 10
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The entry zone of trigeminal sensory root fibers displays a “glial dome” covered by a basal lamina. This zone constitutes the “fibrous cone” of gross descriptions of the root and demarcates a sharp transition from peripheral to central nervous system. The dome consists of closely interwoven astrocyte processes, and appears to be identical to the subpial astrocyte meshwork elsewhere in the central nervous system. In the peripheral portion of the root, axons are surrounded by Schwann cells; those associated with myelin sheaths display distinctive laminar inclusions and pinocytotic vesicles lacking in Schwann cells which surround unmyelinated axons. In the peripheral region, separate and distinct endoneurial and perineurial layers of collagen could not always be identified. In the central part of the root, Schwann cells, fibroblasts, and collagen are absent and from the point of transition, the axons are principally surrounded by astrocytes. Oligodendrocytes are relatively rare in the transitional zone. The axonal transition from central to peripheral, occurs at nodes of Ranvier where the basal lamina of the dome is continuous with the basal lamina of the Schwann cell of the last peripheral internode. Some “islands” of glial tissue are interspersed in the root and ganglion but it was not established if these are completely discontinuous with the central “glial dome.” No ganglion cells have been found in such “islands,” nor in the glial dome.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 11
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Sympathetic ganglia of six Cnemidophorus neomexicanus lizards were fixed by immersion with glutaraldehyde or a combination of glutaraldehyde and paraformaldehyde. All ganglia were post-fixed in 1% osmium tetroxide and embedded in Epon.Neuronal somata and processes of stellate ganglia were ensheathed typically by capsular cell cytoplasm and membranes; however, parts of some processes were invested only by basement membrane. Axo-somatic, axo-dendritic and axo-axonal synapses were observed. Pre- and post-synaptic processes contained 200 Å neurotubules, 100 Å neurofilaments, 225-500 Å presumptive glycogen granules and occasional multivesicular bodies. Presynaptic endings contained 500-700 Å “clear” vesicles and a few dense-cored vesicles of 600-1100 Å.Aggregates composed of 225-500 Å granules, presumably glycogen, were frequently found in peripheral perikaryal positions in intimate association with lipid droplets. Clusters of particles within somata were also found which resembled ribosomes but were not associated with endoplasmic reticulum or vesicular membranous elements. These clusters, perhaps “areticular Nissl substance,” were associated in some instances with fibrillar material or lattice-like granular structures. Abundant and frequently large lipid droplets were observed in proximity to the ribosomal-like particulate material.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 12
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Cells of the vaginal epithelium of ovariectomized mice undergo differentiation in response to estrogen or progesterone injection. Estrogen causes an increase in number of cytoplasmic filaments in the basal and newly formed spinous layer and mucification of surface cells already present. The cells derived from the basal layer under estrogen influence later form a keratinized epithelium. Progesterone causes heightening and mucification of surface cells and invasion of leucocytes into the epithelium without mucification or keratinization of other cells. When estrogen is followed two days later by progesterone, spinous and more superficial cells are lost and replaced my mucous cells derived from the basal layer. Although the fine structure of basal and spinous cell cytoplasm looks identical, the spinous cells do not form mucous cells under progesterone influence. The reverse sequence of progesterone followed by estrogen produces a low epithelium which is then replaced by a keratinized epithelium. The surface mucous cells present during progesterone influence do not keratinize, however. Thus, fine structural features of basal cells typical for the influence of a given hormone do not limit or characterize the potential of daughter cells derived from that layer. Conversely, when hormones are used in sequence, cells which leave the basal layer under the influence of one hormone do not acquire the fine structural features characteristically produced by the second hormone used, even though basal cells are readily altered.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 13
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Fresh-frozen, serial cross-sections of the transversus abdominis muscle of four mature chickens (98 fibers) were examined to determine structural and histochemical characteristics throughout the entire length of skeletal muscle fibers. Fiber diameter and nicotinamide adenine dinucleotide diaphorase (NAD-D) and myosin adenosine triphosphatase (ATPase) activities were used as criteria to classify fibers as Type I or II. Measurements were made at 10 to 22 locations along the length of the fibers. An unimodal distribution of mean fiber diameters ranging from 48 μ to 86 μ was found. Fibers did not appear larger in the belly of the muscle than near the ends. Although small fluctuations in fiber diameter occurred through the length of a fiber, large and small fibers tended to remain relatively large or small at each location. NAD-D activity was either consistently high or low throughout the length of a fiber. Likewise, myosin ATPase activity was either high or low for an entire fiber. It is concluded that skeletal muscle fibers maintain rather uniform structural and histochemical characteristics along their entire length.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 14
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The distribution of acid hydrolytic activities in rat uterine epithelial cells during post-partum involution was examined by light and electron microscopic cytochemistry. Acid phosphatase, β-glucuronidase, N-acetyl-β-glucosaminidase and E600-resistant esterase (cathepsin) increased during the period of involution and the electron cytochemical preparations revealed the lysosomal nature of the acid hydrolytic positive particles visualized by light microscopy.The newly formed particles included primary lysosomes, in the form of Golgi vesicles, vacuoles, and secondary lysosomes such as dense bodies and autophagic vacuoles. This apparent increase in lysosomal activity during uterine involution is in agreement with similar patterns observed in the course of tissue regression in general.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 15
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 164 (1969), S. 253-258 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The mammalian vestibular cilium is described. It is shown to be comprised of nine dense outer fibers and an intermittent central density; this stands in contradistinction to vestibular cilia of non-mammalian species that have been shown to be comprised of nine outer doublet tubules surrounding two central tubules. The possible functional meaning of these observations is discussed.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 16
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The ultrastucture of the canine placental labyrinth has been studied at 40 to 60 days of gestation. Cytotrophoblasts persist in an undifferentiated state throughout gestation, with numerous polyribosomes and little rough endoplasmic reticulum, with expanded cisterns containing flocculent precipitate. Fetal capillaries progressively invade the syncytium, without, however, penetrating the basal lamina. Maternal decidual cells are reported here for the first time in this species. The maternal endothelium is considerably hypertrophied, with many profiles of membranous organelles; but there is consideriable thinning of this endothelium between 50 and 60 days. The interstitial membrane intervenes between trophoblast and maternal endothelium, often forming the thickest part of te barrier at 60 days. the definitive endotheliochorial condition is confirmed; and the thinnest barrier is about 1 μ.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 17
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 165 (1969), S. 61-66 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Adenosine triphosphatase, alkaline phosphatase and acid phosphatase activities of the developing taste buds were observed histochemically in the rat circumvallate papilla.Adenosine triphosphatase activity was already demonstrable in a cluster of cells in the epithelium of the papilla on the first day of birth, when taste buds could not yet be morphologically identified. The cells with the activity are certainly considered as the differentiating taste bud cells. These cells took the form of a bud similar to the adult one about the fourth day of life.Alkaline phosphatase activity was demonstrated first on the superficial cell layers of the epithelium of its apical surface as well as on that of the epithelium in the gutter of circumvallate papilla just after birth. The activity in the gutter then rapidly increased, while the activity of the apical surface abruptly decreased and disappeared on the second day of life.Acid phosphatase was demonstrable only in some cells of the taste bud on the fourth day of life. The final pattern of the activity, which is found in the supranuclear cytoplasm of taste bud cells, was established after the eighth day of life.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 18
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 163 (1969), S. 373-387 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Pregnant mice were hypophysectomized on day 6 and injected subcutaneously with various hormones from days 6 to 9 to establish the minimal requirements for the maintenance of functional corpora lutea. Luteal activity was assessed by the maintenance of pregnancy, weight of embryonic swellings, mean diameter and morphology of corpora lutea, and vaginal histology.Treatment with 2 mg progesterone maintained pregnancy but not corpora lutea in three of five animals. However, the embryonic swellings were significantly (P 〈 0.0005) smaller than those of pregnant control animals. Pregnancy was maintained in all animals when progesterone was combined with 1 μg of estrone. The weights of embryonic swellings and the degree of vaginal mucification in the combined steroid group were similar to those of intact control animals.Treatment with either ovine prolactin, bovine LH, ovine FSH or estrone failed to maintain corpora lutea or pregnancy. Combined injection of prolactin with LH or estrone did not maintain pregnancy or corpora lutea. On the other hand, treatment with 500 μg of prolactin and 200 μg of FSH maintained pregnancy in 12 of 14 animals. All of the aforementioned parameters of luteal activity were comparable to values in intact, control animals.The data indicate that luteal function in the mouse during the early post-implantation period requires a luteotropic complex rather than a single hormone. Prolactin and FSH constitute the minimal luteotropic complex in the pregnant mouse. The luteotropic activity of FSH was not due to its contamination with LH and the effect of FSH was apparently not mediated through estrogen secretion, since pregnancy was not maintained by prolactin and estrone.
    Additional Material: 5 Tab.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 19
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 165 (1969), S. 503-513 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The fine structure of the vasa recta and associated nerves has been studied in the rabbit. Vasa recta were first identified in groups of two or three and were accompanied by small groups of axons. A number of these axons contained granulated, agranular and dense-cored vesicles. As the vasa recta subdivided, the muscle coat consisted of a single layer of smooth muscle cells. Additional large axon bundles were observed accompanying this segment of the vasa recta. Some of these axons also contained granulated, agranular and dense-cored vesicles in varying proportions. Further towards the medulla, smooth muscle cells were replaced by others which had irregular collections of filaments within their cytoplasm. These in turn were replaced by cells having a characteristic band of filaments on their luminal aspects. Nerves were only identified adjacent to those parts of the vasa recta which included smooth muscle cells.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 20
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The effect of anoxia on protein synthesis of developing connective tissue cells was studied by radioautography. Neonatal rats were subjected to anoxia for 20 minutes and were immediately injected with 3 μc/gm body weight of leucine-H3intraperitoneally. They were sacrificed at different intervals after injection. Control animals were handled identically, except that atmospheric air was made available. The heads of the animals were prepared for radioautography in the standard manner. The number of grains was counted over individual fibrobalsts, osteoblasts and chondrocytes of histologically comparable regions, and the data were statistically evaluated.The number of grains per cell was significantly lower in experimental animals sacrificed at one hour after the anoxic treatment and leucine-H3administration. At this time, the average grain number per cell in the experimental animal was less than 50% of the control. The difference was about 20% by four hours and was negligible after 24 hours. The result is interpreted to indicate that the overall protein synthesis in connective tissues is immediately but temporarily suppressed by anoxia and that such suppression might lead to a later functional disturbance among these cells.
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 21
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 164 (1969), S. 141-151 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The technique of organ culture was used to test the effects of various pituitary hormones and norepinephrine on the size of brown and white adipose cells of mice. The hormonal effects were measured by means of histologic analysis and cell width measurements. A concentration of 10 μg/ml of somatotropin (STH), adrenocorticotropin (ACTH) or lipotropin (LPH) was sufficient to reduce the size and content of intracellular lipid in white fat cells while a concentration of 100 μg/ml was required for similar effects on brown fat cells. Norepinephrine, at concentrations of 0.1 μg/ml or 1.0 μg/ml, was sufficient to reduce markedly the size and lipid content of both types of adipose cells. On a molar basis, these hormones acted in a similar range of concentration. The reduction in size and amount of intracellular lipid in brown and white adipose cells by STH, ACTH, and LPH were prevented when combined with 10 μg/ml of insulin. Insulin prevented the lipid depletion effect of norepinephrine in brown but not white adipose cells.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 22
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 164 (1969), S. 153-161 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Onset, frequency and characteristics of the spontaneous calcification which occurs in hearts of mice of the DBA strain have been studied. Calcified myocardial lesions developed as early as 25 days of age. A limited inflammatory response was associated with the fine mineral granules of early lesions. With age, sites of calcium deposition became larger and agranular. There was no evidence of inflammation at such loci and adjacent connective tissue did not increase significantly. In the oldest animals (older than 90 days), fibrous encapsulation of calcified sites was typical. Calcareous epicarditis appeared by 30 days; was minimal (8%) through 90 days, and then rose sharply in incidence (50%) in older animals. Epicardial calcification was restricted to the right ventricle. In general the calcified lesions increased with age and were not related to sex. Incidence of all lesions was 17% by 30 days of age, 80% within 90 days, and 94% in mice older than 90 days.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 23
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 164 (1969), S. 173-184 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Certain members of the extinct reptilian group from which mammals evolved possessed both a dens and an atlas body. Available paleontologic evidence supports the conclusion that the dens evolved as an addition to the atlas body. There-fore, the dens is not homologous with the atlas body as is generally claimed on the basis of supposed developmental evidence. The atlas body is large in the most primitive of living mammals, the monotremes, which also possess a dens of typical mammalian proportions. In metatherian and most eutherian mammals, both a dens and an atlas body remnant of variable size are present. The development of the dens in the Virginia opossum, Didelphis marsupialis, confirms the fact that the dens arises from, but does not replace, the atlas body anlage. The dens evolved as a functional replacement of the atlanto-axial articular processes which were lost when the mammalian atlanto-axial joint became specialized for rotational movement.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 24
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: In order to verify the concept that growth hormone and prolactin are contained in two different populations of acidophils, sections of Bouin-fixed rat hypophyses were stained immunochemically. For this purpose the histochemical demonstration of peroxidase was utilized after sequential application to the tissue section of rabbit antiserum to human growth hormone (or antiserum to rat prolactin) followed by application of peroxidase-labeled sheep antiserum to rabbit gamma-globulin. It was found that growth hormone cells and prolactin cells, when revealed immunochemically, corresponded structurally to cell types that could be differentiated with reasonable certainty in sections stained with the Masson trichrome procedure. When delineated immunochemically, growth hormone cells were larger and more densely arranged in the adult male than in the intact female; they exhibited little change in the female after ovariectomy. In contrast, prolactin cells were large and frequent in the female hypophysis but were small and less frequent in the male and in the female after ovariectomy. By double-staining, growth hormone and prolactin cells were differentiated in the same section. It was concluded that (a) growth hormone and prolactin are contained in, and presumably secreted by, two different populations of acidophils; and (b) the Masson procedure permits a reasonably accurate differentiation of the two cell types.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 25
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 164 (1969), S. 283-289 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: A study of the crura of the diaphragm and their innervation from the phrenic nerve was made in 22 fresh specimens and four embalmed specimens. The arrangement of the musculature was found to be essentially the same as that described by Low in 1907. The portions of the crural musculature passing to the right and left of the esophagus did not decussate anterior to the esophagus as these fibers insert into the central tendon. The right and left phrenic nerves divided into three to five divisions at the pericardiodiaphragmatic angle. Most commonly, these divisions are: anterior, posterior and lateral. If there are more than three divisions involved, there is usually a medial division present and/or a subdivision of one of the other divisions. It is the posterior division or its posteromedial branch which contributes to the innervation of the crura. None of the other divisions contributes to the innervation of the crura. Secondary branches of either the posterior division or the posteromedial branch do not cross to the contralateral side.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 26
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 164 (1969), S. 291-303 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Pregnancy was maintained in Long-Evans rats hypophysectomized on day 1 and injected with ovine pituitary gonadotrophins from day 1 through day 11; and also in rats hypophysectomized on day 6 and injected from day 6 through day 11. For successful maintenance until term, no further injections were necessary after day 11. The combination of hormones considered close to optimal was; mammotrophin 2.5 mg; follicle-stimulating hormone 25 μg; and interstitial cell-stimulating hormone 5 μg injected daily, subcutaneously. Histological examination of ovaries after this regimen (on day 12) revealed interstitial and luteal tissue that was considered normal for this stage; and follicles that were in some cases larger than expected and occasionally with theca luteinization. The ovaries of the rats allowed to go to term and untreated after day 11 showed follicular and interstitial tissue that had regressed and well-stimulated corpora lutea. These findings showed no evidence of placental FSH or ICSH-like hormones; but confirmed earlier reports of a rat placental luteotrophic hormone.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 27
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 163 (1969), S. 31-38 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Using rat's circumvallate papillae, ATPase, alk. Pase and acid Pase of taste buds were observed after the transection of the glossopharyngeal nerve.The taste buds began to disappear after the nerve was cut and were completely lost after ten days. Following the regeneration of the glossopharyngeal nerve, taste buds reappeared from the bottom of the gutters of circumvallate papillae about 25 days after the operation.ATPase was strongly present on the cell membrane of taste bud cells as far as they existed during degeneration and regeneration. Alk. Pase, which is normally localized on the superficial layers of the epithelium overlying the gutters of circumvallate papilla, gradually diminished as the taste buds degenerated and reappeared as the taste buds regenerated; that is, the activity began to diminish three days after the operation, became feeble after ten days and reappeared after 25 days. It is concluded that taste bud cells secrete alk. Pase in the gutters of circumvallate papillae. Acid Pase activity, usually found in the supranuclear portion of taste bud cells, was intensely reactive during degeneration but did not reappear at the early stage of regeneration of taste bud cells.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 28
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 163 (1969), S. 55-57 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Red blood cell count and haemoglobin content for blood of normal adult salamanders, Taricha granulosa, are presented. The RBC count is some 4 × 104/mm3 blood and the haemoglobin level some 4.5 gm/100 ml blood. Some evidence suggesting transient changes in these levels for a brief period immediately following hibernation is discussed.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 29
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 163 (1969), S. 59-65 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: In the human fetal spinal cord, enzymatic activity is demonstrated in neurons of the anterior horn a few weeks before it appears in neurons of the posterior horn. This enzymatic activity is present first in the lumbar segment of the spinal cord and with advancing time gradually ascends cephalad. The presence of acetyl-cholinesterase activity in neuronal cytoplasm in the lumbar segment of the spinal cord coincides with the earliest detectable movement in the lower limbs.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 30
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Attention is directed toward the occurrence of fenestrations or pores in Golgi cisternae of rat and mouse epididymis. There is a gradation in the degree of cisternal fenestration within a Golgi complex. Cisternae at the convex pole are highly flattened and contain few pores, but those near the concave side are distended and exhibit a large number of roughly circular fenestrations 300-600 Å in diameter. Cisternae in the middle of a stack often have an unperforated central portion that is surrounded by a fenestrated and expanded peripheral region. The structure of these Golgi complexes is compared with previous descriptions of Golgi cisternae, particularly the fenestrated cisternae reported in plant cells.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 31
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The endosteal and periosteal diameters of the second metacarpal were measured at the midshaft on radiographs of 62 normal girls at 11.0 ± 0.5 years (pre-menarche) and again at 16.0 × 0.5 (post-menarche). There was an average increase in the periosteal diameter of 10% and an average decrease in the endosteal diameter of 16%, both changes significant at p 〈 0.01. Individual variability was greater for the endosteal than the periosteal surface changes (p 〈 0.01).
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 32
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 163 (1969), S. 71-79 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The capillary network of the papillary layer of the enamel organ was studied with the electron microscope. Mandibular incisor teeth of ten-day old Swiss albino mice were fixed in formaldehyde-glutaraldehyde, postfixed in osmium tetroxide and prestained with 0.5% uranyl acetate prior to being embedded in Epon. Examination of ultrathin sections revealed the presence of fenestrations approximately 500 Å to 700 Å in diameter within the most attenuated regions of the capillary walls. In most instances the fenestrations appeared to be closed by diaphragms usually consisting of a thin membrane with a thicker centrally located button of electron opaque material.The presence of fenestrated capillaries within the papillary region of the enamel organ lends further support to the concept that papillary cells are engaged in transport functions during the maturation of enamel.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 33
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 163 (1969), S. 81-88 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The distribution of intrarenal nervous tissue in a number of species has been examined using a cholinesterase technique. Particular attention has been paid to the relationships between the renal vessels and their nerves. Nerves were detected alongside the major intrinsic renal vessels and formed nerve networks on the external aspects of the arterial tunicae mediae. In all specimens, afferent arterioles were accompanied by nerves the majority of which terminated near the entrance of cortical afferent arterioles into their respective glomeruli; nerves along juxtamedullary afferent arterioles continued across glomerular hila. Relatively few nerves were demonstrated near cortical efferent arterioles while juxtamedullary efferent vessels possessed a rich innervation. Intramural smooth muscle was identified in juxtamedullary efferent arterioles, aglomerular vessels and arteriolae rectae and these vascular segments were accompanied by nervous tissue. Cholinesterase positive nerves could not be detected in the renal medulla.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 34
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 163 (1969), S. 89-99 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Administration of x-rays (4000 r) to lumbar spinal cords of three-day-old rats caused a loss of neuroglia with an inhibition of myelinogenesis, neuronal damage and necrosis. In contrast, the loss of neuroglia following administration of 2000 r is temporary, with myelinogenesis occurring later than normal. Preliminary observations suggested that differences in vascular responses to these two amounts of x-rays might account for these differing fates of the spinal cords; therefore, this investigation was designed to study further the reactions of the intramedullary vessels.Rats, irradiated when three days of age, were killed 1 to 27 days later. Some were killed by perfusion with Monolite Fast Blue BNVSA Paste, a substance retained in the vessels and readily visualized on microscopic examination. The remaining rats were decapitated, and spinal cords were stained by Gomori's method for alkaline phosphatase. The earliest consistent alteration, a decreased number of vessels, was noted seven days following irradiation with either dose. Rats receiving 4000 r had marked losses of blood vessels, vasodilatation and necrosis by 15 days post-irradiation; whereas, a decreased number of vessels was the only change noted in rats irradiated with 2000 r. Alterations in rats receiving 4000 r remained the same or became more severe throughout the study; spinal cords in rats receiving 2000 r returned to normal. These vascular alterations correlate well with the changes in other spinal cord components described previously by this investigator.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 35
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 163 (1969), S. 133-133 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 36
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 163 (1969), S. 101-109 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The fine structure of satellite cells surrounding neurons in the superior cervical ganglia of the rabbit is described. These observations form a basis for the interpretation of fine structural changes which occur in the satellite cells after post-ganglionic section. Early changes consist of an increased number of micropinocytotic vesicles indicating an enhanced uptake of extracellular materials. The mitochondria increase in length and lie close to the plasma membranes, reflecting the increased energy requirement of these cells. Many develop intramitochondrial granules which may be concerned with the transport of water or ions. Later, at the height of chromatolysis, the satellite cells increase their quantity of granular reticulum and many possess agranular sub-surface cisternae whose function may be to direct material synthesised in the satellite cells to the nerve cell surface. Individual layers of satellite cell cytoplasm may slide over one another to accommodate the increased size of the neurons during chromatolysis. The satellite cell processes appear thickened and contain large numbers of fine filaments which may be formed by the breakdown of microtubules.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 37
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Counts of differentiating motor cells over the length of the brachial lateral motor column (LMC) indicate that a large decrease in cell number takes place during the larval period. During the same period an increase in nuclear size of the motor cells occurs with a maximum size attained just following forelimb emergence. Comparison between development of the LMC at the brachial and lumbo-sacral levels indicates a slight lag in brachial LMC development. Cell number remains greater in the brachial LMC than in the lumbo-sacral LMC, but nuclear size is consistently less in the brachial column. Probably no significant change in cell number occurs after metamorphosis, though there is an increase in cell size.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 38
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The ultrastructural effect of progesterone, alone and in combination with estrogen, on smooth muscle cells of the rat uterus was studied. Adult, bilaterally ovariectomized rats were untreated (controls) or treated with either progesterone (1 mg), estrogen (1 μg) or both on three consecutive days.Uterine muscle cells appeared larger and myofilaments more abundant in the progesterone-treated rats than in the other groups of animals. Many micropinocytotic vesicles and several dense bodies were present in muscle cells of control, progesterone and estrogen-progesterone-treated rats. In the progestrone-treated group, smooth muscle cells contained little granular endoplasmic reticulum and few ribosomes and glycogen particles, similar to the controls. Mitochondria were more numerous than in the control animals but similar to those seen in the estrogen or estrogen-progesterone-treated rats. Although an accumulation of granular endoplasmic reticulum, free ribosomes, glycogen particles and extensive Golgi complexes occurred in both estrogen and estrogen-progesterone-treated rats, they were more extensive in the former group.The observations indicate that progesterone alters the ultrastructure of the smooth muscle cells but not to the degree observed following estrogen stimulation. It does not markedly inhibit the effect of estrogen on the fine structure of the uterine smooth muscle cells. These observations support previous biochemical studies on glycogen concentration, RNA and protein synthesis in the rat uterus.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 39
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 40
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 41
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 42
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 163 (1969), S. 359-372 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Various parameters of ovarian activity were determined for the intact or hypophysectomized pregnant mouse, as a baseline to establish the nature of luteotropic hormones in this species.Seventeen per cent of White Swiss mice with a vaginal plug were not pregnant at subsequent stages of gestation. The greatest number of failures occurred between days 12 and 15 of pregnancy, coinciding with the temporary absence of antral follicles and regressive changes in the vaginal epithelium. This suggests that there is a period of transient hormonal imbalance before full placental function is established, which is responsible at this time for the peak in embryonic mortality.Two periods of luteal growth were apparent between days 1 and 4 and 10 to 14 of pregnancy. The first histologic evidence of luteal regression occurred at day 16, correlating with renewed squamous cell proliferation of the vaginal mucosa.There were no significant differences in the number of ova shed on day 1 of pregnancy (11.0 ∓ 0.5 ova) and the subsequent number of embryonic swellings at any stage. Gestation in intact pregnant mice lasted 18 days (n = 2) or 19 days (n = 36). The number of young counted late on day 1 post partum (9.1 ± 0.5) was significantly less than the number of embryonic swellings as a result of maternal cannibalism.Hypophysectomy on day 1 of pregnancy led to rapid histologic degeneration of the corpus luteum. In this feature, the mouse resembled the hamster rather than the rat. Day 10 of pregnancy represented the earliest time at which, at least in some animals the pituitary could be removed and pregnancy continue. Following hypophysectomy from day 11 on, luteal activity, continuation of pregnancy, fetal and placental weight and vaginal histology were comparable to intact, pregnant mice. This is similar to the hypophysectomized rat in the latter half of pregnancy but differs from the situation in the hamster.On the basis of the present findings and results in the following paper, it appears likely that the mouse placenta, in addition to secreting a prolactin-like hormone, also produces other gonadotropins.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 43
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: In order to evaluate pathological alterations of the inclusion bodies of Type II cells in the mammalian lung, normal morphology of inclusions has been studied in rabbit, sheep, rat, mouse, dog, hamster and guinea pig. In rabbit, and sheep the fetal development of the inclusions has also been investigated.After a standard method of fixation, three different types of inclusions have been found in the species studied. The first type of inclusion is seen in the fetal rabbit, fetal sheep, dog, rat and mouse. In these species the inclusions contain heavily osmiophilic, coarsely lamellar material. In all of these species, the inclusions seem to form from an interaction between tubulo-vesicular bodies, located near the Golgi apparatus, and multivesicular bodies. These are certain variations in the appearance of the inclusions among these species.The second type of inclusion is seen in the hamster and guinea pig, in which lightly osmiophilic material accumulates during the maturation of the inclusions. In these species, a granular dense body seems to occupy an important role in the formation of the inclusion bodies.In the third group, the postnatal rabbit and sheep, the inclusions appear vacuolated.It is postulated that the seemingly inconsistent results of the acid phosphatase activity of inclusions in several species may indeed be a reflection of species differences in the mode of formation of inclusion bodies.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 44
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 165 (1969), S. 127-139 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: In the course of ultrastructural studies on the cerebellar cortex of young rats a cilium was found in some Purkinje cells. The shaft, which is 1800 to 2000 ÅR thick, has an internal portion inside the cell body and an external one projecting into the neuropile. The basal body is consistently located near the Golgi apparatus. Rootlets with periodic cross-striations (interperiod distance of 525 ÅR) are attached to the basal body. The size and characteristics of the Purkinje cell cilium and its constituent parts compare with similar structures in the cilia of other cell types of the cerebellar cortex. Whether all or only some of the Purkinje cells have a cilium has not yet been determined. Possible functional roles of these organoids are considered.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 45
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 165 (1969) 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 46
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 165 (1969), S. 329-341 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Myoid cells of the thymus of hibernating frogs were examined by electron microscopy. This cell type is represented by immature, fully-developed and degenerating cell forms. The immature forms are mainly located at the surface, whereas the developed ones are found in the inside of the thymus. A peculiar type of immature cell containing no thick filaments, but possessing a rich sarcoplasmic reticulum among the thin filaments is described. The developed myoid cells have a myofibrillar apparatus showing regular cross-striations. The myofibrils are arranged in concentric layers around the nucleus. The proposed role and origin of myoid cells and their probable connection with myasthenia gravis are discussed.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 47
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 164 (1969), S. 35-45 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The fine structure of testicular interstitial cells of lion and three-toed sloth showed an abundance of agranular endoplasmic reticulum, mitochondria and lipid droplets. Mitochondria were usually small and rod to rounded in shape, but large bizarre forms, such as triangular, oval and rounded with tubular and lamellar cristae were also observed. Mitochondria with lamellar cristae occurred in lion, but not in three-toed sloth. These cristae were highly developed and appeared unique for the testicular interstitial cells. Both small and large mitochondria closely adhered to lipid droplets which were found singly or in clusters. The stored lipid droplets probably serve as a major source of substrate in androgen biosynthesis. The possible role of agranular endoplasmic reticulum in androgen biosynthesis is suggested.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 48
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 164 (1969), S. 47-65 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Thymic rudiments from mouse embryos varying in gestational from 10-13 days were examined by light and electron microscopy in an effort to learn the origin of thymic lymphocytes. Lymphocytes were recognized and distinguished from mesenchymal cells and thymic epithelial cells by their round shape, larger nucleoli, and high concentration of cytoplasmic ribosomes and dearth of endoplasmic reticulum. No lymphocytes could be found at the earliest stage of development of the third pharyngeal pouch  -  at approximately ten days' gestational age. They first appeared in the mesenchyme surrounding the third pouch at a later stage, (approximately 11 days' gestation), but in most cases did not appear in the thymic epithelium until the parathyroid and thymus began to differentiate at approximately 12 days' gestation. No cells were seen that appeared to be transitional between lymphocytes and epithelial or mesenchymal cells and it was concluded that these observations support the hypothesis that lymphocytes first reach the thymic parenchyma by immigration from the surrounding mesenchyme. Most of the lymphoctyes found in the region of the thymus at these stages of development were large lymphocytes. The origin of these early lymphocytes remains unknown.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 49
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 164 (1969), S. 67-77 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Eruption of permanent premolars in puppies was studied by implanting stainless steel wires through the mandible to stop eruptive movement well before the teeth were to appear in the oral cavity. Histologic and radiologic comparisons of operated with normal (unoperated) sides disclosed that the eruptive pathway forms for the impacted teeth although their eruptive movements were effectively prevented. This clearly shows that the mechanism of eruption pathway formation is not dependent upon direct and continual pressure on the bone from the erupting teeth. The genesis of pathway formation appeared to be the same for the impacted teeth as for the controls i.e., through continued ostetoclasis in the margin of the stalk of the dental follicle.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 50
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 164 (1969), S. 79-99 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Globule leucocytes (GL) first noted by Weill ('19) in the intestinal tract of certain mammals, have been described, since, in many species. The classification and function of these cells, however, is still under discussion. We have studied GL in the cat using histochemistry at light and electron microscopic levels. In this species, GL are concentrated in the intestinal mucosa; they are found less frequently in the gallbladder and only rarely in other organs. GL are usually located between the epithelial cells of the gut, seldom in the underlying lamina propria. They appear to be migratory cells of mesenchymal origin, representing an independent cell population. GL are characterized by large eosinophilic globules in their cytoplasm. The globules contain strongly basic proteins associated with phospholipids and neutral mucosubstances which, in the colon, are encased in a capsule of sulfated mucosubstances. At the ultrastructural level the typical globules consist of a dense, homogeneous matrix enclosed by a single membrane; they show, however, great variation in their morphologic appearance, reflecting, possibly, different stages in the process of accretion and utilization of their content.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 51
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The repair of hemopoietic bone marrow following evacuation of the tibial or femoral cavity of the rat was sequentially studied with the light microscope. A stereotyped train of histologic events occurred. These were capillary invasion of the cavity, appearance of primitive mesenchymal cells, osteoblastic proliferation, cancellous bone formation, development of sinusoids, reappearance of hemopoietic tissue and resorption of cancellous bone. The studies suggest that restoration of marrow sinusoids takes place only in the interstices of cancellous bone. Mechanical disruption of the sinusoidal system is one method of triggering cancellous bone formation. The cancellous bone which appeared after injury was thought to be produced by endosteal osteoblasts and osteoblasts derived from cells residing in normal hemopoietic tissue. Localized radiation of the tibia followed by mechanical disruption of hemopoietic tissue demonstrated that cancellous bone production and the repair process were unimpaired by 1,000 r but were completely blocked by 4,000 r. This would imply that the cell which can differentiate into an osteoblast is resistant to 1,000 r.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 52
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 164 (1969), S. 127-139 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Calcium-deprivation of three weeks duration causes marked degenerative changes in the shell gland mucosa of the laying hen. The height of the columnar epithelium decreases, but the secretory granules in the columnar epithelial cells and the cilia on the apical cells of the columnar epithelium are preserved. The tubular glands decrease in size and number. The microvilli surrounding the tubular gland lumen disappear. The cytoplasm of the tubular gland cell contains autophagic vacuoles (cytolysomes) and large pigment accumulations, presumably lipofuscin. The changes are due to dietary calcium deficiency, apparently mediated by decreased sex steroid secretion and decreased stimulation of the oviduct. These changes are totally reversible, since the fine structure of the shell gland mucosa of calcium-deprived hens returned to a normal laying diet does not show any signs of degeneration and is identical with the fine structure of the shell gland mucosa of the laying control birds.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 53
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 164 (1969), S. 259-265 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Histological preparations from skin of the Australian black snake, Pseudechis Porthyriacus have revealed myelinated nerve fibers terminating in several structures. The commonest of these, the simple “End Button,” was seen in the epidermis from all regions of the body. An end organ, the “touch corpuscle” occurred in the epidermis of the upper and lower lip, while in the dermis of the upper and lower jaw an encapsulated structure, the “club corpuscle” was frequently encountered. These more complex structures could not be revealed in skin of the trunk where only “end buttons” were seen.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 54
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 164 (1969), S. 305-316 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The effect of fluoride on the physical properties of bone was investigated with the aid of an Instron materials tester. Two groups of weanling female rats, one on an adequate (0.6%) calcium diet and the other on a low (0.1%) diet were given the following dosage levels of fluoride (as NaF) in their deionized drinking water over a fifteen and one-half week period: 3.4 ppm, 10.0 ppm and 45.0 ppm. In the adequate calcium group a significant increase in flexibility in the rat femur was found only at the 45.0 ppm dosage level. This was not offset by a significant decrease in strength. In the low calcium group a similar significant increase in flexibility appeared at the 10.0 ppm dosage level as well as the 45.0 ppm, but a significant decrease in strength at the two dosage levels was observed. These were in direct relation to the amount of fluoride given.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 55
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 164 (1969), S. 317-332 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The fine structure of the adrenal medulla of Macaca mulata monkey was studied after double fixation with paraformaldehyde and OsO4; other fixations were employed for comparison. Only one cell type was seen. The cytoplasm contained an uneven distribution of chromaffin vesicles which were either spherical or elongated and 0.1-0.3 μ in diameter. There were two principle vesicle categories: dark and compact forms which comprised the majority (about 80%) and lighter, mostly larger, vesicles with a finely granulated appearance. The Golgi apparatus was often seen in relatively clear areas where few other organelles were present. Vesicles, 0.1-0.2 μ in diameter, which stained heterogeneously were also observed. They were few in number and always associated with the Golgi apparatus. The chromaffin cells lay in close proximity, but in no specific relationship, to thin-walled blood vessels. Nerve endings which were partially embraced by Schwann cells were in synaptic relation to the chromaffin cells.These findings are compared with previous observations in other mammals and the nature of the dark and light vesicles is discussed.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 56
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Skin fragments implanted subcutaneously into syngeneic mice remained viable and showed cyclic hair growth over a period of months. Embryonic skin differentiated into the various layers of epidermis and corium, producing sebaceous glands and hair follicles. Implants from both embryos and newborn displayed normal hair pigmentation with appearance in metanagen of the yellow bands characteristic of the agouti hair for that genotype. Follicles were observed to pass through the various hair growth stages into a resting period and then later to re-enter the growth cycle. There were departures from normal hair growth, however, with some follicular disorganization, especially in the newborn implants, and with areas showing mixed growth stages instead of the follicular synchronization characteristic of the intact mouse.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 57
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Ligatures were placed unilaterally on the ductus deferens (experiment 1), on the ductus deferens and the middle of the corpus epididymidis (experiment 2) and on the ductus deferens, corpus epididymidis and ductuli efferentes (experiment 3) in 39 rabbits. The untreated contralateral side served as a control. Vasectomy alone appeared to have no effect upon the testes, or upon spermatozoa accumulated during a period of 12 weeks proximal to the ligature. In experiment 2 severe disruption of spermatogenesis occurred. Diameter of the seminiferous tubules decreased, testes atrophied, and the stages of the seminiferous epithelium were abnormal and often not classifiable. Some regeneration was observed after 12 weeks.In experiment 3 there was only a moderate and more transient disturbance of spermatogenesis. This occurred in spite of an accumulation of fluid in the testis due to the ligature on the ductuli efferentes, which prevented testicular effluent from reaching the caput epididymidis. These results suggest that when testicular effluent is allowed to reach the caput, but nonresorbed residues are prevented from further transport (experiment 2), a feedback to the testis occurs which is more harmful than that produced by preventing tubular contents from leaving the testes.
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 58
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 164 (1969), S. 359-377 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Autonomic nerves in the rat, mouse, guinea-pig and cat were vitally stained with methylene blue and examined electron-microscopically after fixation in potassium permanganate. Dense patches in the axoplasm and swelling of mitochondria were found in the stained axons. By controlling the pH and saturating with oxygen, only cholinergic axons were stained at pH 6.5-7.0 whereas at pH 5.0-5.3 both cholinergic and adrenergic axons stained. Similar results were obtained by immersion in a bath at controlled temperature as with close arterial perfusion of dye into pelvic viscera. The intermediate pH at which adrenergic axons begin to stain could not be determined. However, as a means of identifying cholinergic axons, particularly those sectioned at a level where no agranular vesicles occur, M B staining is unreliable because not all cholinergic axons respond. In sympathectomized rat iris lacking adrenergic axons altogether, some of the persisting cholinergic axons remained unstained. Experiments with M B thiocyanate and M B chloride in phosphate-citrate or succinate-HCl buffer indicated that purity of dyestuff was less important than pH control. Methods for electron-microscopical demonstration of acetylcholinesterase are more useful for distinguishing cholinergic axons especially if adrenergic nerve vesicles are I reserved in the same preparation by the initial fixation.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 59
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 164 (1969), S. 379-390 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Intramuscular nerve branches are covered by a complex of epithelial sheaths which decrease in thickness and complexity as the nerves branch to reach ultimate termination of neuromuscular junctions. The outermost layer (epineurium) of intramuscular nerves is composed of thin processes of fibrocytes lacking basement lamina and associated with parallel bundles of collagen. Internal to this layer are two or three layers of interdigitating perineural cells covered with basement lamina. The innermost layer is frequently incompletely covered by basement lamina within the perineural sheath. Schwann cells covered with basement lamina enclose myelinated and unmyelinated axons.In the region of neuromuscular junctions, the basement lamina of Schwann cell processes merge with the myofiber basement lamina. Thin perineural cell processes form a bell-shaped covering which does not reach the muscle basement lamina. The relationships of myelin to the axon as the terminal axon emerges from the Schwann cell closely resembles the relationships of these structures in nodes of Ranvier.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 60
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 61
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 163 (1969), S. 389-401 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: In this experiment actinomycin D was used to explore the action of the wound epidermis on underlying tissues during limb regeneration. In axolotl forelimbs the skin was removed from the elbow to the shoulder. Skin from the right limbs was soaked for three hours in actinomycin D (5.0 or 10.0 μg/ml 0.6% NaCl). For controls, skin from left limbs was soaked in 0.6% NaCl for the same period of time. Each piece of skin was orthotopically replanted, and both limbs were amputated through the treated skin, proximal to the elbow. After an initial healing period, the control limbs regenerated normally. Except for a slightly paler color, limbs bearing actinomycin-treated skin were indistinguishable from the controls, both grossly and histologically, during the first week following amputation. While the control limbs formed early blastemas, no grossly visible evidence of regeneration was apparent in the experimental limbs, but histologically some dedifferentiation was occurring. Normally three to four digits were seen in the control regenerates before blastemas appeared on the experimental limbs. By 35-40 days blastemas had appeared on most experimental limbs. These developed very rapidly, and within a short time many of them had attained levels of development close to the controls. Actinomycin D temporarily suppresses formation of the apical epidermal cap and the subsequent aggregation of dedifferentiated cells into a blastema. When the effect wears off, an apical cap forms and the dedifferentiated cells quickly organize into a blastema and begin to differentiate.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 62
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 163 (1969), S. 403-425 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Developing myotubes in skeletal muscle tissue from the limbs of larval newts have been examined with respect to the ultrastructure and sequence of events accompanying myofibril formation. A population of filaments having a diameter of 100 Å is found to occur throughout and beyond the period of myofibrillogenesis. This population is in addition to developing actin and myosin filaments and probably does not contribute directly to myofibril formation. Rather it may represent a cytoskeletal network which ultimately becomes principally disposed around and at right angles to older myofibrils at the level of their Z-bands.Assembly of thick and thin filaments into myofibrils seems to occur, in this muscle, predominantly near the periphery of the cell with registration of these components into A-, I-, and Z-bands being accomplished as they assume progressively more internal locations. Z-bands appear to develop by coalescence of Z-bodies which in turn are earlier related to skeins of fine filamentous material which commonly occupy the most peripheral cytoplasm of these and other mesenchymally derived cells. Fine structural details of these skeins, Z-bodies, and Z-bands have been analyzed with regard to the several prevailing concepts of Z-band architecture. An hypothetical sequence for myofibril formation and Z-band differentiation is presented which takes into account several observations and relates them to the looping filament configuration previously proposed for mature Z-band structure.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 63
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The morphology of differentiating heterophils and eosinophils of the slender salamander, Batrachoseps attenuatus, was studied with electron microscopy. Enzymes of the granules of both cell types appear to arise from the Golgi cisternae. An agranular stem cell was not observed and the least differentiated cell type encountered was an “early” promyelocyte having small granules which are morphologically distinct from mature granules of either the heterophil or eosinophil series. Heterophil myelocytes and later stages contain only one population of granule which is fibrous in content. Eosinophils likewise possess but one type of granule; the granules are larger than those of heterophils, have a homogeneous content, and lack the crystallin core so characteristic of mammalian eosinophils.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 64
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 163 (1969), S. 443-451 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Anatomists and gynecologists have debated the existence of a rectovaginal septum in the human female. In this investigation the connective tissue between the vagina and rectum has been reexamined by both dissection and light microscopy in four specimen types. Specimens studied and compared totaled 143 and represent an age range of from eight fetal weeks to 100 years. Included were specimens from patients with different degrees of parity and both normal and abnormal pelvic visceral support.It has been shown that a definite rectovaginal septum exists in the human female and it is probably the homologue of the male rectovesical septum. The septum is well formed by the fourteenth fetal week and consists of a thin vertical sheet of dense connective tissue which is translucent in the fresh state. In the coronal plane the septum parallels the sacral curvature. It also curves posterolaterally to become fused with the parietal endopelvic fascia. The septum extends inferiorly from the rectouterine peritoneal pouch to the perineal body. It is usually adherent to the posterior aspect of the vaginal connective tissue, capsule. This adherence, together with the difficulty in identifying it histologically may explain why the existence of the rectovaginal septum has been denied.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 65
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 163 (1969), S. 453-471 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The heads of three-day old rats were irradiated with a single dose of 200 r x-ray and the animals were killed afterwards at intervals ranging from ten minutes to five days. Necrosis in the external granular layer of the cerebellum was evident by the fourth hour and the pyknotic cells increased in number up to 12 hours after irradiation. Between 24 to 48 hours all the pyknotic cells disappeared and the width of the layer was drastically reduced. By the third day after irradiation the external granular layer began to increase in width, and by the fourth day it was indistinguishable from normal. In adults of this group the cerebellum appeared structurally normal. In another experiment the cerebellum of rats was exposed from birth onward to 200 r on five successive days. In the animals killed immediately or one day after the last radiation session the external granular layer was totally or subtotally eradicated. In the animals surviving for four days the external granular layer reappeared over many regions of the cerebellum, and by the sixth day after irradiation it was present over its entire surface. In the latter group in animals that survived to 30 and 90 days of age the cerebellum contained a large, though subnormal, population of granule cells, indicating that the reconstituted cells were able to differentiate. These results suggested that the proliferative matrix of the postnatally developing cerebellum may be endowed with regenerative capacity.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 66
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 163 (1969), S. 473-482 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: This investigation was undertaken to examine the effects of aging on alkaline phosphomonoesterase (Alk Pase) activity in the adrenals of a highly inbred strain of C57BL/10 male mice. A total of 140 male mice were assigned to seven main groups and sacrificed at 1, 4, 8, 12, 16, 20 and 24 months of age for biochemical and morphologic evaluations of adrenal Alk Pase activity. The biochemical findings indicated that while aging may result in a decrease of serum and liver Alk Pase activity, enzyme activity in the adrenals of male mice increases to a maximum level at approximately eight months of age and subsequently decreases at each successive age level. The histochemical findings revealed that the highest concentrations of enzyme activity occurred in the fascicular and reticular zones of sexually mature male mice. There were no major variations in zonal distribution with advancing age. Electron microscopy showed Alk Pase activity along membranes of cortical cells and within the subendothelial space.The progressive increase in Alk Pase enzyme activity up to eight months of age, and the subsequent fall in activity during senescence as well as its absence in the adrenals of female mice provides further support for a role of androgen in mobilizing cortical alkaline phosphomonoesterase activity.
    Additional Material: 17 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 67
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The ultrastructure of Kupffer cells of canine liver has been studied and described. Kupffer cell nuclei were elongated and irregular in shape having a prominent double nuclear envelope containing scattered ribosomes on the outer membrane. Mitochondria were small, relatively scarce, and contained few mitochondrial granules. Elements of endoplasmic reticulum were scarce and highly irregular in form. In general, the cytoplasm was less dense than hepatocytes and contained fewer organelles which were less compactly arranged than in hepatocytes. Microbodies were not observed in any of the Kupffer cells studied. The cytoplasm contained various numbers of large phagosomes but consistently contained numerous dense cytosomes of various sizes. The most striking finding was that many cytosomes and phagosomes contained large numbers of short microtubules approximately 350 Å in diameter which were arranged in clusters and were associated with membanes.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 68
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Endobronchial lavage was performed on eight smokers and eight nonsmokers. Centrifugation of lavage fluid produces a sediment consisting of two layers, a lower compact brown layer containing cells and an upper flocculent white layer. The brown layers from the smokers were greater in volume than those from the nonsmokers. Macrophages constituted about 93% of the cells from the smokers and about 63% of the cells from the nonsmokers. These data suggest that more free macrophages occur in the lungs of smokers than nonsmokers. In addition, many of the macrophages obtained from the smokers were filled with cytoplasmic inclusions. The volumes of white layers from the smokers were smaller than those from the non-smokers. One white layer obtained from a nonsmoker was examined in a Wilhelmy balance and proved to be surface-active. This may suggest that surface-active material, pulmonary surfactant, is reduced in lavage fluids from smokers.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 69
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The morphology of platelets fixed in acetaldehyde and postfixed in osmium tetroxide was compared with that of platelets fixed in glutaraldehyde followed by osmium tetroxide. Many platelets became rounded after acetaldhyde fixation. Membranes, granules and mitochondria were well preserved and cytoplasmic microfilaments became apparent. Very dense granules were obvious after acetaldehyde fixation alone and after acetaldehyde/glutaraldehyde/osmium tetroxide triple fixation, but not after acetaldehyde/osmium tetroxide double fixation. Microtubules were never seen. Intracytoplasmic vacuoles assumed exaggerated prominence. The authors relate the alterations in the vacuolar system and changes in platelet shape to a loss of cytoplasmic microtubules. They suggest that the changes are induced, in part by the low temperature of fixation, and in part by the weak fixative action of the aldehyde.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 70
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 163 (1969), S. 517-524 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The vascular supply to the skin of pigs was studied in animals perfused with the dye, monastral fast blue. The arrangement of the blood vessels in control animals was in three plexuses, similar to that in other mammals but with the middle plexus poorly developed.A litter of pigs was divided into two groups at the age of 12 days and one group was raised in a warm room and the other in a cold room. The pigs were killed at age three months when the warm room was 35°C and the cold room 5°C. A quantitative evaluation of the blood supply to the skin of the experimental animals revealed that the pigs from the warm room had more blood vessels in the skin than those raised in the cold; the difference was statistically significant (P 〈 0.01). In the case of pigs from a second litter reared at 35°C and 20°C, no significant difference in the number of blood vessels was detected.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 71
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 72
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 165 (1969), S. 107-108 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 73
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 74
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 165 (1969), S. 109-112 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 75
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 165 (1969), S. 113-115 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 76
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 165 (1969), S. 117-119 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 77
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 165 (1969), S. 121-124 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 78
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 165 (1969), S. 125-125 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 79
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 165 (1969) 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 80
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The development of the pineal organ in the newt Taricha torosa has been studied utilizing cell counts and radioautography following single injections of tritiated thymidine. Embryos injected two weeks before hatching (series I) demonstrated a grain distribution pattern in the pineal organ and its underlying proliferation zone characteristic of continuous availability of isotope. Larvae injected at hatching (series II) or two weeks after hatching (series III) displayed the expected pulse label pattern for these same regions. With the possible exception of some mitosis insituin the youngest organs, pineal cells originate from a mitotically active cell population which comprises the pineal proliferation zone. After cell division some daughter cells migrate into the pineal organ, moving into the posterior part of the organ during the prehatching period, while from hatching onward the predominant migration is into the anterior part of the organ. Both the pineal photoreceptors and supportive cells arise in this manner with labeled cells of both types found in all three series, but in decreasing numbers from the youngest to the oldest series.Cell counts disclose an approximate ten-fold increase in the number of cells within the pineal organ from embryonic to adult stages, but the rate of cell addition slows with increasing age. Both photoreceptors and supportive cells show this increase in number with the photoreceptor population being maintained at a constant 14%-18% of the total pineal population over this entire five-year period.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 81
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 165 (1969), S. 257-328 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 82
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 165 (1969), S. 355-361 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The growth changes in the cranial base of the rabbit between one and 20 weeks after birth have been investigated. The basicranial axis and its anterior extension completed some 50% of their growth during this period in contrast to the posterior extension which had completed 70% before one week. The sphenoethmoidal angle underwent no statistically significant changes despite a decrease in the spheno-maxillary angle indicating that the face became disposed below rather than directly anterior to the braincase. The foramino-basal angle decreased progressively during the period of investigation.The pattern of growth in the linear dimensions of the rabbit cranial base is essentially similar to that already established in man and subhuman primates. The angular changes, however, differ from those in primates where the spheno-ethmoidal and foramino-basal angles follow more closely the developmental patterns of the braincase and facial skeleton.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 83
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 165 (1969), S. 363-377 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The effect of antigenic stimulation with pertussis and sheep red blood cell antigens was studied in the rat popliteal lymph nodes. H3-leucine was used to assess protein metabolism in the various cell types of the reactive lymph nodes following both primary and secondary stimulation with these antigens. Radioautographs of tissue smears were used to make quantitative and qualitative assessments of the extent of intracellular protein synthesis. In addition, changes in the cellular populations of various lymphoid cell lines were determined utilizing differential cell counts and total cells present in the reacting nodes. These data were used principally to assess the role of the lymphocytic cells in the ontogeny of the immune response.Large and medium lymphocytes responded to antigenic stimuli by increasing intracellular protein synthesis. This newly synthesized protein most likely reflected the requirements of large and medium lymphocytes for growth, division and the production of immunoglobulins. The small lymphocytes, as a group, showed only minimal changes in intracellular protein synthesis following antigenic stimulation.The responses by large and medium lymphocytes and plasma cells were qualitatively similar following primary and secondary stimuli. The differences in these responses appeared to be reflected by the number of cells involved and by the temporal sequence in which the cells appeared.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 84
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: An electromyographic investigation of the activity of the paired genioglossus and geniohyoid muscles of twenty-six human subjects during deglutition revealed a general pattern of muscular activity involving an initial build-up, gradual summation, and tapering of electrical potentials during swallowing of both saliva and water. There is an observable difference in the pattern of swallowing of individuals within a group and among the individual swallows of a single subject. There are longer periods of electrical activity during a saliva swallow than during a water swallow. The type of bolus also seems to affect the pattern of activity in the individual muscles as well as the length of time that they are working. The geniohyoid muscles do not appear to begin their activity with the genioglossus muscles but rather lag behind and they do not appear to be active for as long. Both pairs of muscles appear to remain active during and after the time that the bolus has passed the area of the laryngopharynx. A period of electrical silence occurs prior to the characteristic burst of activity associated with a swallow. This appears to be the result of an active inhibition.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 85
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Light and electron microscopic examination of serial transverse sections of interalveolar septa of hamster lung have demonstrated a virtually continuous connective tissue sheet of variable thickness in the interalveolar septum. This central sheet, like connective tissue elsewhere, is composed of ground substance in which are immersed cells, collagen and elastin. A capillary network lies on each surface of this sheet. The capillary basement membrane is continuous with the central sheet but in contrast to it is thin, uniform and devoid of cells, collagen or elastin. Capillaries on one surface frequently anastomose directly through the septum with capillaries on the opposite surface.The central sheet is shown to be virtually continuous despite frequent thinning. Whenever cells, collagen or elastin occur in the interalveolar septum, it is always in the central sheet. The efficiency of this arrangement in combining optimum gas conductance with adequate mechanical support is pointed out.The central sheet is also continuous with the perivascular connective tissue cuff and thus may serve as an important route of extracellular fluid transport to the perivascular lymphatics.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 86
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 163 (1969), S. 555-561 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: A line sampling method was used to determine the relative volumes of various cell types in the mucosa of the body of the rat stomach. The technique was simple to perform, reproducible and independent of many variables inherent in previous cell counting techniques. Weights of the various cell types were calculated from stomach weights, relative mucosal volumes and cell concentrations in the mucosa. In the five animals studied, gastric body mucosa average 40% of the stomach weight and 1.8 gm per kg of rat. Parietal cells averaged 40% of the gastric body mucosa weight and 0.7 gm per kg of rat. There was no significant variation in parietal cell concentration throughout the gastric body mucosa of the rat. Chief cells averaged 18% of the gastric body mucosa and 0.3 gm per kg of rat. Up to three-fold variations in chief cell concentration occurred with highest concentrations near the greater curvature and in proximal areas of the body mucosa. Mucous surface cells, mucous neck cells, and connective tissue made up 22, 9, and 7% of the body mucosa respectively and showed only minor varitions in concentration.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 87
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Representative pieces of labyrinth of near-term chorioallantoic placentas from three species of Dipodomys and one species of Microdipodops were fixed in glutaraldehyde and osmium tetroxide and examined with the electron microscope. The barrier separating the fetal and maternal blood streams in the labyrinth was found to consist of (1) the fetal endothelium and its associated basal lamina (basement membrane), (2) a single layer of trophoblast which is cellular rather than syncytial, and (3) the maternal endothelium and its associated basal lamina. Thus the chorioallantoic placenta appears to be of the endotheliochorial type in these rodents rather than hemochorial or hemoendothelial as previously suggested.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 88
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 163 (1969), S. 563-573 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The deciduomal response to uterine trauma in pesudopregnant hamsters has been studied. Trauma was effected in one uterine horn by leaving a sterile silk thread in the lumen, pulling a thread through the lumen, injecting oil or air into the lumen or crushing the horn with a hemostat. At autopsy the traumatized and untraumatized horns were weighed separately and examined histologically for a deciduomal reaction. The day of greatest uterine sensitivity was found to be day 4 of pseudopregnancy, and maximal deciduomal growth (mean uterine weight ± S.E. = 2582 ± 279 mg) occurred by day 8. No significant further increase in uterine weight was seen by day 9, and by day 10 separation of the deciduoma from the myometrium had begun. Leaving a thread in the uterine lumen produced a significantly greater uterine weight by day 8 than with any other stimulus, but good deciduomal responses (uterine weights of 1679 ± 245, 1255 ± 156 and 1192 ± 219 mg) were induced by oil injection, pulling a thead through the uterus and crushing the uterus respectively. A poor reponse was produced by air injection. At no particular time during day 4 of pseudopregnancy was it possible to demonstrate a peak of uterine sensitivity using as a traumatic stimulus either oil injection or pulling a thread through the lumen. In this respect hamsters differ from both rats and mice, which have well defined peaks of maximal uterine sensitivity.
    Additional Material: 6 Tab.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 89
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Hearts of mice were fixed in osmium tetroxide, embedded in Epon, sectioned for electron miscroscopy and stained with uranyl acetate. Various regions of these hearts were examined for neuromuscular relationships. Unmyelinated nerve bundles with partial Schwann cell investment, frequently are present within the perivascular spaces of capillaries and arterioles in the myocardium of all regions studied. Neuromuscular junctions are located in the myocardium of the right atrium, the inter-ventricular septum and the outer ventricular walls. Neuromuscular junctions seem to be absent from the myocardium of the apex. Previously reported neuro-effector relationships within various tissues are discussed in relation to the findings of this study.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 90
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 163 (1969), S. 587-593 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Skin samples from 16 genera and 19 species of bats were studied histologically. These and review of the literature revealed musculi arrectores pilorum composed of smooth muscle present only in Megachiroptera and complex cutaneous striated muscle systems best developed in Microchiroptera. Smooth muscle arrectores of the mid-dorsal neck skin of Hypsignathus monstrosus are described in detail and resemble those of generalized structure in other mammalian Orders. Shoulder pouches of the epaulet bats (Epomophorus and Epomops) contain compound smooth and striated muscle systems for eversion of hair tufts and pouch respectively. An evolutionary trend within the Chiroptera of reduction in smooth muscle arrectores and replacement by integumentary striated muscle fibers is suggested.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 91
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 163 (1969), S. 595-601 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: In combination with biochemical determination of cholesterol in testes, cholesterol was studied histochemically in the testicular interstitial cells of dd strain mice from 5 to 63 days.Cholesterol is found in interstitial cells at five days and cholesterol positive interstitial cells increase significantly from 7% at 5 days to 47% at 21 days (17% at 7 days, 21% at 10 days and 31% at 14 days) and thereafter gradually to 58% at 63 days (50% at 28 days, 54% at 35 days, 56% at 42 days, 58% at 49 days and 57% at 56 days).The cholesterol concentration of testes (μg of cholesterol per mg testicular weight) remains at low values before 42 days (1.33 at 5 days, 2.12 at 7 days, 1.89 at 10 days, 2.18 at 14 days, 2.16 at 21 days, 1.63 at 28 days and 2.09 at 35 days) but it increases rapidly at days 42-49 (2.21 at 42 days and 5.16 at 49 days) and then it is almost unchanged (5.47 at 56 days and 5.18 at 63 days).From these results it is suggested that (1) at five days testicular steroid could be elaborated and (2) the capacity of interstitial cells to produce testicular steroid elevates actively from 42 to 49 days.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 92
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Teeth undergoing resorption were dissected from the maxillae of frogs and prepared for electron microscopy using standard methods and methods designed to minimize demineralization. The resorptive cells overlapped one another, forming palisades. A central ruffled border was surrounded by a relatively structureless cytoplasmic “seal.” Crystals of mineral were present in the folds of the ruffled borders, and demineralized collagen fibrils were seen in the adjacent matrix in sections treated to prevent demineralization. The surfaces of the cells opposite the ruffled borders displayed numerous small villi.The cytoplasm of the resorptive cells contained a variety of lysosomes in various stages of development, mitochondria, scattered vesicles of endoplasmic reticulum and free ribosomes. Well developed Golgi zones were frequently seen, and occasional sections passed through centrioles. Two centrioles were commonly seen in such sections, but never more. Occasional patches of cytoplasm contained mitochondria and rough-surfaced endoplasmic reticulum, resembling “blast” cell cytoplasm.We concluded that the resorptive cells of several mineralized tissues of various vertebrates are morphologically, indistinguishable.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 93
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 164 (1969), S. 15-33 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The lateral limit of the subarachnoid space, where nerve roots enter and leave, forms the subarachnoid angle. This is an important site of transition for nerve sheaths. Here the perineurium of peripheral nerve leaves the surface of the nerve and extends between the dura mater and the arachnoid. The perineurium is therefore open-ended with respect to the subarachnoid space. The central perineurial extension is histologically the same as perineurium in some areas but in others forms a layer of hydrated cells without basement membranes. These lie in close apposition with the outermost cells of the arachnoid membrane. At the subarachnoid angle the arachnoid membrane may either reflect onto the root sheath or be attached to it by punctate junctions. The root sheath covers the nerve roots as they pass through the subarachnoid space. It is composed of loosely arranged cells bound by punctate junctions. Its intercellular spaces may contain connective tissue fibrils. A single basement membrane separates it from the endoneurium. The histological structure in the region of the subarachnoid angle is consistent with clinical evidence implicating the endoneurium of nerve trunks as a pathway for the transmission of infection from the periphery to the central nervous system.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 94
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The effect of thymectomy at different ages in C3H mice on development of circulating leukocytes and cells of marrow, spleen and lymph nodes has been analyzed. Regardless of the age at which thymectomy is performed depression of numbers of circulating lymphocytes is produced. Thymectomy at birth did not affect significantly the relative number of lymphocytes in the marrow during the first few weeks of life, later they fell to low levels. Thymectomy at four weeks was followed by prompt reduction in relative numbers of lymphocytes in the marrow.After reaching six weeks of age, neonatally thymectomized mice showed a high proportion of monocytes in the marrow. Neonatal thymectomy and thymectomy at two weeks of age reduced the number of eosinophils in the marrow.Neonatal thymectomy inhibited development of lymphocytes in the spleen, whereas thymectomy later in life produced only transient depression of lymphocytes in this organ. In addition, neontal thymectomy decreased the relative numbers of small lymphocytes in the lymph nodes. This was associated with drastic depletion of lymphocytes in the deep cortical regions of the nodes.
    Additional Material: 4 Tab.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 95
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: There are constant morphological differences between the bony pelves of adult males and females that constitute a sexual dimorphism. A convexity along the caudal border of the ischiopubic ramus and a bony projection bordering the pubic joint are present only in the male. The pubic joint in the male is a typical symphysis, whereas in the female it consists of an interpubic ligament interconnecting the coxal bones. The ligament contains an abundance of elastic fibers interspersed among collagenous fibers. The disparity between the size of the pelvic birth canal of the pregnant bat and the single full-term fetus is so great that during parturition the interpubic ligament stretches to more than 15 times its original length.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 96
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 164 (1969), S. 391-401 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The manner of insertion of intrafusal (IF) fibers of muscle spindles has been studied in serial histological sections of spindles from hindlimb muscle of the cat, opossum, mouse, rat, bat and gibbon. Four basic types of IF terminations in relation to the capsule were recognized: (A) direct insertion into the capsule; (B) strong lateral attachments to the capsule, though the fiber may continue beyond the capsule; (C) termination in the connective tissue beyond the limits of the capsule; (D) free extension of the fiber beyond the capsule. Bag fibers in all species generally extended beyond the capsule. The pattern of chain fiber insertion, however, varied with the species. Most rat spindles, for example, followed the D pattern, those in the cat pattern A, and the gibbon chiefly A and B. The bearing of these findings upon a postulated mechanism by which spindles may detect intramuscular pressures, as changes in length, is discussed.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 97
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Comparative light and electron microscopic study of nuclei in rat trigeminal neurons identified two structures which are the ultrastructural equivalent of the paranucloelar structure and the accessory body of Cajal, two nucleoplasmic structures previously demonstrated in other neurons by light microscope silver staining methods. Ultrastructural evidence indicates that the dense component of the nucleolus is converted into the paranucleolar structure, which then detaches from the nucleolar surface to lie free in the nucleoplasm as the accessory body of Cajal. The cytochemistry, ultrastructure, and antimonate reactivity of the paranucleolar structure and accessory body were identical. Both structures lacked cytochemically demonstrable DNA, RNA, or basic protein.The neuronal nuclei also contained Feulgen-positive sex chromatin bodies that adhered to the nucleolus, the nuclear membrane, or to both of these structures in specimens of female but not male rats. The ultrastructure and antimonate reactivity of these bodies closely resembled that of heterochromatin clumps but differed markedly from that of the paranucleolar structures and accessory bodies.Additional structures characterized ultrastructurally included patches, granular bodies, and flakes. These structures, like the paranucleolar structure and the accessory body of Cajal, are apparently unique to nuclei of neurons. Cytochemical methods showed that the patches contained basic protein but no nucleic acid.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 98
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 164 (1969), S. 479-487 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Rats, having been given a single subcutaneous injection of testosterone propionate at four days of age, exhibited polyfollicular ovaries, extensive stratified squamous uterine metaplasia and persistent vaginal cornification (90-95% of the smears contained cornified cells) after adulthood was attained. If early androgentreated rats were blinded at 22 days of age the number of vesicular follicles in the ovaries of adults was greatly diminished, the incidence and extent of stratified squamous metaplastic lesions were suppressed and the number of vaginal smears containing cornified cells was reduced by about 50%. The effects of blinding, however, were negated by pinealectomy illustrating that the suppressive influence of light deprivation on reproduction was mediated by way of the pineal gland.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 99
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Photophores in Porichthys notatus are bioluminescent organs composed of a lens, photogenic tissue, and an underlying reflector. Nerves penetrate through the reflector and branch, free of their Schwann cells, among the photogenic tissue elements. The tortuous pathways of axons have been followed by electron microscopical serial sections. After giving off occasional terminal branches, the axons continue into pockets of connective tissue which penetrate among the photogenic, supportive, and lens cells. There they form tuft-like clusters of nerve endings, some of which contain dense-cored vesicles. None of these endings was seen to penetrate the basal lamina and make direct intercellular contact with either a supportive cell or a photogenic cell. From these observations, it is concluded that the nerves end outside of the basal lamina surrounding photogenic, supportive, and lens cells. Adhesion sites, which may indicate chemical synaptic contact, were occasionally observed among these neural components.The photophores of the California Porichthys notatus often bioluminesce following intraperitoneal injection of the fish with epinephrine. It therefore appears from experimental, anatomical and fine structural evidence, that these luminescent organs are innervated and controlled by the sympathetic nervous system.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 100
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Photophores in Porichthys notatus consist of a cellular lens, an underlying area of photogenic tissue and a deep ensheathing reflector. Lens cells exhibit a dense compacted filamentous cytoplasm. Their processes interdigitate and desmosomes exist along their borders. The photogenic tissue, richly supplied by blood vessels, consists of two main cell types: (1) a presumed “photogenic” cell; and (2) a supportive cell. Photogenic cells are characterized by a highly vesiculated cytoplasm and peripheral microvilli. Frequently, they exhibit lamellar membranous whorls. Some whorls display cytoplasmic cores with vesicles. Many of these vesicles communicate via pores with an extracellular channel that envelops the cells. Supportive cells contain cytoplasmic filaments and extend processes around the photogenic cells. Except for isolated desmosomal contact points, a wide extracellular channel intervenes between supportive and photogenic cells. A prominent basal lamina separates supportive cells from the surrounding connective tissue. The strongly birefringent reflector is composed primarily of cells containing guanine crystals. The crystals lie stacked in groups, each membrane-bounded crystal being separated from its neighbor by an intervening layer of cytoplasm. Such an arrangement produces constructive interference and accounts for the high reflectivity of this multilayered structure. Possible relationships of the above structural features and the production of light are discussed.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...