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Digitale Medien

Expression of two Trichoderma reesei endoglucanases in the yeast Saccharomyces cerevisiae (1987)

Panttilä, Merja E. ; André, Lars ; Saloheimo, Markku ; [weitere]

New York, NY [u.a.] : Wiley-Blackwell

Yeast 3 (1987), S. 175-185

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Details

ISSN: 0749-503X

Schlagwort(e): Cellulases ; Endoglucanases ; Trichoderma reesei ; Saccharomyces cerevisiae ; Cellulolytic yeast ; secretion ; Life and Medical Sciences ; Genetics

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie

Notizen: The cDNA copies of the two endo-β-1,4-glucanase genes, egl1 and egl3, from the filamentous fungus Trichoderma reesei were expressed in yeast Saccharomyces cerevisiae under the control of the yeast phosphoglycerate kinase gene promoter. Active EGI and EGIII enzyme was produced and secreted by yeast into the growth medium. The recombinant EGI enzyme was larger and more heterogeneous in size than the native enzyme secreted by Trichoderma, due to differences in the extent of N-glycosylation between these two organisms. The morphology of the yeast cells producing EGI or EGIII was clearly different from control strain.

Zusätzliches Material: 6 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/yea.320030305

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