Digitale Medien
Oxford, UK
:
Blackwell Publishing Ltd
The @journal of eukaryotic microbiology
11 (1964), S. 0
ISSN:
1550-7408
Quelle:
Blackwell Publishing Journal Backfiles 1879-2005
Thema:
Biologie
Notizen:
SYNOPSIS. Sulfate-S35 uptake by intact Euglena cells, as well as incorporation of sulfate-S35 into the insoluble fractions of the cells, was partially inhibited by adding iodoacetamide simultaneously or ten minutes before radioactive sulfate. The identification of S-cysteine acetamide and S-glutathione acetamide showed the presence of glutathione and cysteine in the soluble pool of Euglena but methionine, homocysteine, and S-adenosylmethionine were not found during these experiments. An unidentified radioactive peak was eluted by 2 n HCl during column chromatography of the soluble fraction of Euglena cells. The major compound in this peak was identified as L-cystine on the basis of its infra-red spectrum, paper and column chromatographic characteristics and optical rotation. The use of iodoacetamide permitted the independent demonstration of cysteine and cystine in the soluble pools by eliminating oxidation artifacts.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1111/j.1550-7408.1964.tb01732.x
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