ISSN:
0935-6304
Keywords:
Capillary GC
;
Uncoated precolumns
;
Solvent peak
;
Porosity of capillary surfaces
;
Deactivation methods
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Many uncoated precolumns, and to a lesser extent separation columns, strongly retain some of the solvent. Retarded release of such solvent elevates the baseline after the solvent peak and causes ugly “humps” of eluted solvent as the temperature is increased. The problem is probably a result of retention by a porous surface, e.g. surfaces obtained by leaching or hydrothermal treatment of capillaries prior to silylation. It is assumed that other problems with capillary columns can be explained by the same mechanism, including: a kind of adsorption including apolar compounds, and “ghost” peaks as well as “memory” effects. Fused silica capillaries are superior to glass, but even for these special procedures are required in order to achieve thorough deactivation of the internal surface without introducing porosity.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240131203
