ISSN:
0935-6304
Keywords:
Liquid Chromatography, HPLC
;
Stationary phase carrier silicagel analysis and cleaning treatment
;
0.3% metal traces may interfere
;
Elution by boiling 1-12 N HCl does not attack C18 bonding in reverse phase material, extracts metals sufficiently
;
Effective phase quality test by hop-α-acid chromatogram
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Trace elements in different silica gels are determined by neutron activation analysis. The presence of 20 elements in the ppm range and of 15 elements in the ppb range is established in all silica gels; even in spherical material probably obtained via an organosilicium starting material. Removal of these elements by acid treatment before and after derivatization to a reversed-phase is studied. This is only partially effective. The resulting HPLC phases are, however, much better in cases where trace elements are detrimental. Even octadecyl derivatized silicagel can withstand boiling in 12 N hydrochloric acid without loosing significant amounts of bonded organic material.
Additional Material:
2 Tab.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240020311
