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Isomers of uroporphyrin free acids separated by HPLC (1979)

Wayne, A. W. ; Straight, R. C. ; Wales, E. E. ; [et al.]

Weinheim : Wiley-Blackwell

Journal of High Resolution Chromatography 2 (1979), S. 621-622

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ISSN: 0935-6304

Keywords: Uroporphyrin isomer separation ; HPLC uroporphyrin free acid ; Uroporphyrin isomers in urine ; Chemistry ; Analytical Chemistry and Spectroscopy

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Notes: A synthetic mixture of uroporphyrin isomers I, II, III and IV as free acids in the synthetic ratio of 1:1:4:2 was resolved by reverse-phase HPLC using a C0:PEL (ODS) 37-50 micron precolumn and a Micro Bondapak C18 analytical column eluted with acetonitrile (4%) in phosphate buffer (pH 6.95). Clinically important I and III isomers of uroporphyrin were readily resolved directly from acidified urine as porphyrin free acids.

Additional Material: 2 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/jhrc.1240021008

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