ISSN:
0935-6304
Keywords:
Liquid chromatography, HPLC
;
Small bore columns
;
Systematic performance studies with tabulated results
;
Seven compounds separated in less than 10 s
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Using a specially designed column system, we have systematically investigated the effect of mobile phase velocity on column efficiency. The performance of small bore columns operated at different linear velocities of mobile phase was examined for three different types of injection system. Using the value of H∞/u or n∞/trº as a criterion of a high speed separation, we calculated values of n/trº for different solutes according to the equation \documentclass{article}\pagestyle{empty}\begin{document}$ {{\rm n}\mathord{\left/ {\vphantom {{\rm n} {{\rm t}_{\rm r}^ \circ }}}\right. \kern-\nulldelimiterspace} {{\rm t}_{\rm r}^ \circ }} = {{{\rm n}^\infty } \mathord{\left/ {\vphantom {{{\rm n}^\infty } {{\rm t}_{\rm r}^ \circ }}} \right. \kern-\nulldelimiterspace} {{\rm t}_{\rm r}^ \circ }}\left({\frac{{1 + {\rm k'}}}{{{\rm k' + }\beta }}} \right)^2 $\end{document}; the results obtained are in agreement with the experimentally determined values. These systematic investigations culminated in the separation of seven compounds in less than 10 s; the respective chromatogram is shown.
Additional Material:
8 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240041106
