ISSN:
0935-6304
Keywords:
Thin-layer chromatography, TLC
;
Temperature effect on retention
;
Martin's equation
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
A study has been undertaken on the effect of temperature on retention characteristics in thin-layer chromatography (TLC) with low-volatility mobile phases (MP). It is shown that temperature variations in TLC in melts bring about variations in both the relative retention values and, in some cases, in the order of migration of the chromatographic zones across the layer. The variation in the capacity factor k' with temperature agrees well with Martin's equation. To explain the temperature dependence of Rf one must, in general, take into account the variations with temperature in both the partition coefficient and the phase ratio. To describe the variation in Rf with temperature in TLC with low-volatile MP one can use an approximate equation in which In Rf is a linear function of 1/T. The experiments indicate that temperature is a major factor in TLC in melts.
Additional Material:
7 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240060409
