ISSN:
0935-6304
Keywords:
Liquid chromatography, HPLC
;
Normal phase
;
Serum
;
Retinol
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
A method for the quantitation of retinol in serum by normal phase high performance liquid chromatography is described. The mobile phase consists of a hexane/diethylether/methanol mixture with spectrophotometric detection. The sample preparation is similar to that described by other authors and involves protein precipitation followed by extraction and direct injection onto the chromatograph. Adequate sensitivity is achieved using 200 μl samples. The internal standard chosen is α-naphthol because of its spectral similarities to retinol and its greater stability than the commonly used retinyl esters. The method has been successfully applied to the clinical evaluation of a number of malabsorption syndromes for which it is preferred to the measurement of total carotenoids.
Additional Material:
7 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240070804
