ISSN:
0935-6304
Keywords:
Liquid chromatography, LC
;
Exclusion chromatography
;
Glass microcolumns
;
Protein association
;
Surfactants
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
The application of microcolumn exclusion chromatography to the determination of equilibrium and kinetic association constants for proteins is described. Taking phospholipase A2 as an example, the relationship between association and enzymatic activity was investigated. A procedure for determination of the critical concentration of micelle formation (CCM) in surfactants was developed.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240090211