ISSN:
0935-6304
Keywords:
Liquid chromatography, HPLC
;
Selectivity
;
Polycyclic aromatic hydrocarbons, PAH
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
An empirical test is described for the evaluation of column selectivity in reversed-phase liquid chromatography. Using a test mixture of three polycyclic aromatic hydrocarbons (PAH), overall column selectivity toward PAH was assessed for over 20 different commercial C18 columns. Retention behavior was correlated to phase type (i.e., monomeric and polymeric surface modification chemistry) for custom synthesized phases. A classification scheme is proposed in which commercial C18 columns are grouped into three classes based on retention behavior: monomeric-like, polymeric-like, and intermediate phase selectivity toward PAH. Correlation of retention behavior of the test mixture with the separation of PAH mixtures and with more general column properties (e.g., phase thickness) is discussed.
Additional Material:
2 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240110505
