ISSN:
0935-6304
Keywords:
Capillary gel electrophoresis
;
Surface modification
;
Separation of oligonucleotides
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Polyacrylamide gel filled capillaries for electrophoresis can be improved in performance and stability by a specially optimized and reproducible production procedure. After surface pretreatment as described in this contribution, gels polymerized in capillaries are stable for long series of routine separations. Bubbles formation during gel polymerization can be avoided. The procedure is compared with those described by other authors. Separations of different oligonucleotide samples illustrate the quality of the gel capillaries obtained. The peak symmetry achieved proved to be better than that obtained without surface pretreatment.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240130908
