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Matrix-assisted laser desorption/ionization mass spectrometry of synthetic oligodeoxyribonucleotides (1992)

Parr, Gary R. ; Fitzgerald, Michael C. ; Smith, Lloyd M.

New York, NY : Wiley-Blackwell

Rapid Communications in Mass Spectrometry 6 (1992), S. 369-372

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ISSN: 0951-4198

Keywords: Chemistry ; Analytical Chemistry and Spectroscopy

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Physics

Notes: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry has been used to mass analyze a variety of synthetic oligodeoxyribonucleotides ranging in size from 6 to 30 nucleotides. Molecular ions were observed almost exclusively, with little or no fragmentation and generally minor contributions from multiply charged species. Mixtures of poly d(T)12-30 with low picomole amounts of each component gave well resolved peaks. These results are encouraging for the concept of DNA sequence analysis by mass spectrometry.

Additional Material: 6 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/rcm.1290060603

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