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The high performance liquid chromatography of enzyme systems relating to purine and pyrimidine metabolism: an overview (1990)

Perrett, David ; Simmonds, H. Anne

New York, NY : Wiley-Blackwell

Biomedical Chromatography 4 (1990), S. 267-272

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ISSN: 0269-3879

Keywords: Chemistry ; Analytical Chemistry and Spectroscopy

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Medicine

Notes: Purines and pyrimidines are now routinely separated by HPLC. By careful selection of chromatographic conditions which match the expected changes in hydrophobicity and/or ionic nature of the substrate and products most enzymes of the purine and pyrimidine salvage pathways can be routinely and accurately determined. Ion-paired reversed-phase systems are often the most advantageous. The relevance of such assays to biomedical analysis including their role in the diagnosis of inborn errors of metabolism is stressed.

Additional Material: 4 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/bmc.1130040613

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