ISSN:
1040-0397
Keywords:
Modified electrodes
;
Reverse-phase
;
HPLC
;
Proteins
;
Peptides
;
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
An indicator electrode, in which a film containing Ru oxides with cyano-crosslinks comprised the active surface, was used in an amperometric detector for ion-exchange and reverse-phase high-performance liquid chromatography (HPLC). The latter used a C-18 column and an eluent of 0.04 M citric acid, 0.1 M KH2PO4, 0.1% trifluoroacetic acid, and 20-80% acetonitrile. Oxytocin, isotocin, vasopressin, tocinoic acid, myoglobin, and ribonuclease A were separated and determined with detection limits at the 1-3 pmol level. The response to ribonuclease A, as a typical example, was linear over the range of 0.050-2.5 μM, with a slope of 15 nA/μM at 0.94 V (vs. Ag/AgCl). A single preparation of the electrode was generally stable for 3 weeks, as long as the analyte concentrations were below 10 μM.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elan.1140050806
