ISSN:
1040-0397
Keywords:
Enzyme reactor
;
Flow injection analysis
;
Simultaneous determination
;
Glucose
;
Uric acid
;
Cholesterol
;
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Glucose, uric acid, and cholesterol in serum were assayed simultaneously in a flow injection system by using a 16-way switching valve and a parallel configuration of three immobilized enzyme reactors. Nafion film-coated and Nafion/cellulose acetate film-coated platinum electrodes were used to selectively detect the hydrogen peroxide generated enzymatically in the enzyme reactors, without any interferences from electroactive species such as L-ascorbate and proteins present in the serum. The signal current was linearly related to the glucose concentration in the range of 5-700 mg/dL, to the urate concentration in the range of 1-80 mg/dL, and to the cholesterol concentration in the range of 5-100 mg/dL. The precision (coefficient of variation) was better than 2.8% for the simultaneous assay of the three species in human control serum. The assay speed was up to 32 samples per hour.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elan.1140070209
