ISSN:
1040-0397
Keywords:
Biosensor
;
NADH
;
Meldola Blue
;
Redox polymer
;
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
A water-insoluble modified methyl-siloxane polymer which held 9-dimethylaminobenzo[a]phenoxazine-7-ium (Meldola Blue) units, was synthesized, characterized, and used to drop-coat solid graphite electrodes. The electrocatalytic oxidation of the nicotinamide adenine dinucleotide (NADH) in the polymer could be described by the Michaelis-Menten type of kinetics as seen from steady-state measurements in 0.25 M phosphate buffer, pH7, at 0 mV (vs. SCE). The electrocatalytic properties of the redox polymer was evaluated using the film thickness as a major variable. The theoretical model found to describe the NADH oxidation at the redox polymer modified electrode, is presented. A maximum NADH sensitivity of 40 μA cm-2 mM-1 at a coverage of 0.5 μg cm-2 was indicated.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elan.1140071005
