ISSN:
1052-9306
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Desorption chemical ionization (DCI) mass spectra of a series of phorbol di- and monoesters have been determined by mainly using ammonia and isobutane as reagent gases. The applicability of this mass spectrometric method in structural characterization of this class of compounds is discussed. The DCI (NH3) mass spectra of phorbol diesters provided immediate confirmation of the molecular weight via an abundant adduction, [M + NH4]+. The DCI (iso-C4H10) mass spectra more clearly afforded useful structural information with regard to the constitutent fatty acid attached to C-12 or C-13 and deacyloxy moiety. Therefore, a combination of the mass spectra with both reagent gases allows characterization of phorbol esters. The intensities of peaks in the DCI (iso-C4H10) mass spectra can be used to distinguish the phorbol-12-monoesters from the 13-monoesters. It was found that hydrolysis of the phorbol 12,-13-diesters followed by DCI mass spectrometric analyses enabled the location of the longer-chain acyl moiety at either C-12 or C-13 in the parent alcohol.
Additional Material:
7 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bms.1200200108
