ISSN:
0030-493X
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
A rapid method was developed for the analysis or polar conjugated bile acids by combined packed column super-critical fluid chromatography/mass spectrometry. Direct coupling of the column to the mass spectrometer was effected with a modified thermospray interface operated in the filament-on mode. Maximum sensitivity for the bile acid conjugates was achieved by recording the negative ions generated in the ionization process. The effects of vaporizer and source temperatures, repeller voltage and discharge conditions on the bile acid response were investigated. The mass spectra obtained for the common glycine and taurine conjugates yielded only the [M - H]- pseudo-molecular ions. In contrast with the taurine derivatives, the glycine forms produced a weak ion current and exhibited broad hands. The applicability of the technique is illustrated with a sample of human bile.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/oms.1210271119
