ISSN:
0749-1581
Keywords:
NMR
;
1H NMR
;
Vassopressin derivatives
;
Chemical exchange
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Proton exchange rates between the solvent H2O and the amidic protons of a nonapeptide (1-desamino-8-D)-argininevasopressin in aqueous solution were determined by using selective nuclear magnetic double-resonance methods. Transverse magnetization, the magnitude of which depends on the chemical exchange rate and the relaxation time T1, is transferred via doubly selective homonuclear Hartmann-Hahn mixing to a scalar coupling partner. With respect to well established two-dimensional experiments, this one-dimensional alternative has two advantages: it is much less time consuming and, as a true benefit, it is even applicable when two signals which are in exchange with the same compound (solvent) are overlapping. The evaluation of amide proton exchange rates in the investigated peptide led to the assumption of an intramolecular hydrogen bond.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/mrc.1260330207
