ISSN:
0173-0835
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
The stability with time of Immobiline solutions and of pre-cast Immobiline gels has been investigated, for the former by high pressure liquid chromatography and for the latter by focusing a set of marker proteins. Temperature seems to affect these chemicals most: whatever degradation occurs at 20°C within a few weeks' time, is operative at 60°C within a few hours' time. These buffers are altered by pH in different ways: acidic Immobilines (pKs 3.6 and 4.6) are extensively degraded around neutrality (pH 6) while basic species (especially pK's 8.5 and 9.3) are massively destroyed in alkaline solutions (pH 9). Acidic pH values (pH 3-4) ensure maximum stability for all Immobiline chemicals. In terms of medium-term storage of pre-cast gels, wet matrices exhibit least degradation if titrated to pH 4. Formic acid is the best titrant since it can be efficiently removed during the focusing step with formation of the narrowest salt-front at the anode. Dry matrices have much longer stability (〉2 months) but should be stored with traces of humidity to prevent the cracking and peeling-off from the plastic support which occurs at zero relative humidity.
Additional Material:
7 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.1150060404
