ISSN:
0887-6134
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
In negative ion chemical ionization mass spectra of prostanoid pentafluorobenzylester(PFB)/methoxime(MO)/ trimethylsilylether(TMS) derivatives [M-PFB]- is the most abundant fragment ion. Collisionally activated decomposition (CAD) spectra of this ions show nearly only fragmentation of trimethylsilanol (TMSOH), (CH3)2Si=CH2, carbon dioxide and methanol. CAD spectra of [M-PFB]- ions of 3,3′,4,4′-deuterated PGE2 and 6-oxo-PGF1α PFB/MO/TMS derivatives fragmentation of methanol and deuteromethanol (CH3OD) is observed. The ratio of CH3OH/CH3OD is about 3:1 (PGE2) and 9:1 (6-oxo-PGF1α) respectively. This loss of deuterium in the internal standard needs to be considered when employing the isotope dilution technique in quantitative prostanoid analysis and tandem mass spectrometry.
Additional Material:
1 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bms.1200181015
