ISSN:
1075-4261
Keywords:
guanosine 5′-diphosphate
;
guanosine 5′-triphosphate
;
magnesium
;
vibrational spectroscopy
;
Raman spectroscopy
;
FTIR
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Physics
Notes:
Raman and infrared spectra were examined for guanosine 5′-diphosphate (GDP) and guanosine 5′-triphosphate (GTP) in aqueous solution. The vibrational modes were assigned on the basis of isotopic frequency shifts and relative intensities in the Raman and infrared spectra. The observed frequency shifts on 18O isotope labeling made it possible to identify the bands from each phosphate group (α, β, γ). Frequency shifts were observed as Mg2+ complexes with GDP and GTP. The results suggested that Mg2+ binds to GDP in a bidentate manner to the α, β P · · O bonds and in a tridentate manner to the α, β and γ P · · O bonds of Mg·GTP. The results indicate that structure of Mg2+ coordinated to GTP in aqueous solution differs somewhat to that found for Mg·ATP. © 1998 John Wiley & Sons, Inc. Biospectroscopy 4: 219-227, 1998
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
