ISSN:
0006-3525
Keywords:
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
An electron microscopic investigation on the structure of fibrin is reported. Fibrin morphology was investigated in a wide variety of experimental conditions, and by carefully controlled staining procedures. Two main band patterns A (230-Å-spaced main dark bands) and B (230-Å-spaced main light bands) are observed for stained fibrin; it is shown that the former results from the superimposition of both positive and negative staining, and the latter is given by positive staining. By suitable denaturation experiments, it was found that the fiber is composed of a regular alternation of lose and dense regions along the axis.We have assumed that the monomer of fibrin is described by the three-nodular model of Hall and Slayter, as supported by recent investigations. The monomers are arrayed according to a head-to-tail sequence along the fiber, and to a staggered lateral association. This model accounts for all the experimental observations, and predicts well the high-resolution band pattern of fibrin. It further agrees with the results of a recent work on the early stages of the fibrinogen-to-fibrin conversion.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bip.1975.360141110
