ISSN:
0006-3525
Keywords:
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Intensity-fluctuation laser-scattering spectroscopy measurements have been reported for the DNA isolated from bacteriophage φ29. The cumulant method of data analysis gives well-defined weighted average decay frequencies. Available theoretical models are shown to yield good values for the diffusion coefficient and longest intramolecular relaxation time for low-molecular-weight DNA's but not for higher molecular weight species. The temperature dependence of the apparent diffusion coefficient exhibits drastic oscillations in the helix-coil transition region. The number of these oscillations is consistent with independent measurements of uv hyperchromicity measurements and suggests that φ29-DNA is composed of at least three regions of differing G + C content.
Additional Material:
2 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bip.1977.360160209
