ISSN:
0006-3592
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Strains of the green algae Scenedesmus obliquus, Chlorella vulgaris, and C. pyrenoidosa 7-11-05, and of the blue-green algae Fremyella diplosiphon, Plectonema calothricoides, Phormidium luridum, and Synechococcus lividus have been mass cultured autotrophically in essentially pure D2O. Closed Lucite rocking-box units containing 5 liters of culture media can produce 1 to 2 g. (dry weight) per day of fully deuteriated algae or of fully deuteriated, fully 15N-substituted algae. Racks for six such culture units are readily constructed from commerically available parts. Operation has been routine and essentially trouble-free. The algae produced have proved to be an excellent source of completely isotopically substituted metabolites.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bit.260040304