ISSN:
0006-3592
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Constant current voltametry was shown to be a suitable method for evaluation of electrodes containing immobilized oxidative enzymes as catalysts. The half-cell potential for the oxdidation of D-glucose at constant pH was measured at a series of constant dc currents, using glucose oxidase immobilized in a polyacrylamide gel-platinum gauze matrix as catalyst. Over the range studied the potential versus current data were linear and could be extrapolated to give the open-circuit half-cell potential at zero current. The difference between the measured potential and the open-circuit potential at a constant current was indicative of the loss in useful voltage due to concentration gradients within or adjacent to the enzyme electrode assembly. The relationship between the open-circuit potential and the pH of the reaction mixture was linear with a slope of -0.056, in good agreement with the predicted slope of -0.061.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bit.260130504
