ISSN:
0006-3592
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
The penicillinase yield YP/X (U/g cell) of a transformant Bacillus stearothermophilus CU21 (pLP11) in continuous culture of LGP broth at 44, 47.5, and 50°C, respectively, depended not only on temperature, θ (°C), but also on dilution rate, D (h-1), in a peculiar fashion that could not have been realized if the product were from the gene on the chromosome rather than the plasmid. The gene dosage effect could account for the unusual dependence of YP/X on θ and D, because the mode of YP/X as a function of D at a given temperature resembled that of plasmid content Cp (mg plasmid/g cell) vs. D at the same temperature. In other words, Cp, corresponding to the copy number of plasmid per grams of cell, could be controlled by either θ, D, or both in this instance. However, when the gene expression efficiency, ε (U/ng plasmid), was plotted versus Cp, the effects of θ and D on the expression became indistinct.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bit.260270522
