ISSN:
0006-3592
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
A simple procedure is developed to process experimental data from plasmid maintenance studies of recombinant cells in a chemostat with nonselective medium. This procedure, based on the model proposed by Imanaka and Aiba, provides quantitative information on the rate of plasmid loss and the difference in the specific growth rate between the plasmid-carrying and plasmid-free cells. The performance of the proposed method is evaluated through simulation studies. In addition, the method is applied to a set of previously reported experimental data. The two-parameter model, together with the estimated parameter values, provides an excellent fit to the experimental data.
Additional Material:
8 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bit.260330410
