ISSN:
0006-3592
Keywords:
steriod Δ1-dehydrogenation
;
immobilization
;
Arthrobacter simplex
;
polyurethane
;
organic media
;
thermostability
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Whole Arthrobacter simplex cells entrapped in κ-carra-geenan or in two types of polyurethane foam, or adsorbed on silanized Celite, were tested for the Δ1-dehydrogenation of hydrocortisone and its derivatives in organic media. Catalytic activity and stability levels were evaluated for the immobilized cells in buffer with 2.5% (vol/vol) methanol, and in three buffer-saturated solvents (n-octan-1-ol, n-decan-1-ol, and chloroform). The addition of glutamate to the immobilization support stabilized the activity of the immobilized cells in the tested organic media. The system with polyurethane (HYPOL6100)-entrapped cells (with coimmobilized glutamate) in n-decan-1-ol provided the highest long-term activity levels. Several factors involved in the polyurethane-entrapment procedure were also studied. © 1992 John Wiley & Sons, Inc.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bit.260400918
