ISSN:
0730-2312
Keywords:
c-AMP
;
G-proteins
;
Ki-ras oncogene
;
proliferation
;
transformation
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
Notes:
Rat kidney (NRK) cells infected with a temperature-sensitive mutant of the Kirsten sarcoma virus were arrested in the G0/G1phase of their cell cycle by incubation in serum-deficient medium at a p21-inactivating temperature of 41°C. These quiescent ts K-NRK cells were then stimulated to transit g1 and initiate DNA replication by lowering the temperature to 36°C, which rapidly reactivated p21 Reactivating the viral Ki-RAS protein by temperature shift led to an increase in adenylate cyclase activity in early G1 phase. The Ki-RAS protein increased the sensitivity of adenylate cyclase to guany1 nucleotides by a mechanism that seemed to involve inactivation of the enzyme's inhibitory G1regulatory protein.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jcb.240330203
