ISSN:
0730-2312
Keywords:
growth inhibition
;
primary hepatocytes
;
liver epithelial cells
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
Notes:
Improvements in the purification of a hepatic proliferation inhibitor (HPI) from adult rat liver have yielded a product that has an inhibitory activity 1,000-fold greater than previously reported. The growth inhibitory activity, which could be eluted from SDS-PAGE at 17-19 kilodaltons (kD), was compared to that of transforming growth factor beta (TGF-β). The ID50 of the HPI preparation in Fischer rat liver epithelial cells was 50 pg/ml (2.5 pM) compared to a value of 260 pg/ml (10.4 pM) obtained for pure human TGF-β. Both inhibitors also modulated the stimulation of DNA synthesis in primary hepatocytes by either epidermal growth factor or a growth stimulatory activity prepared from serum of hepatectomized rats. The ID50S of HPI and TGF-β in these cells were 250 pg/ml and 40 pg/ml, respectively. In contrast to TGF-β the growth inhibitory activity of HPI was unaltered in the presence of an antibody raised against TGF-β. The possible mechanism of action of HPI is discussed.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jcb.240350405
