ISSN:
0749-503X
Keywords:
Bromocresol purple
;
killer toxin
;
Saccharomyces cerevisiae
;
fluorescence staining
;
yeast
;
Life and Medical Sciences
;
Genetics
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
Notes:
A method is described for detecting yeast cells with plasma membrane damage, based on cell staining with bromocresol purple (BCP) which has a convenient fluorescence after entering the cells at pH below 5·2. The method was used to determine the activity of Saccharomyces cerevisiae pore-forming killer toxin K1 in commonly used lethal units. The BCP test is rapid and as precise as the plating test.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/yea.320091107